Advantage and disadvantages of fluorescence microscope?

Answer 1

It is easier to view because it already has a light source. *Basically you can not use living tissue with Electron Microscopes, but only Light compound ones. "Because electron microscopes require a vacuum to operate samples must e preserved & dehydrated before they are placed inside the microscope. Meaning that living cells cannot be observed with electron microscopes, only with the light ones."

Answer 2

photobleaching

and also some limitations arise from

1) the availability of target specific antibodies. If they are not commercially available you have to make them which is time and money consuming.

2) Specificity of the antibody. Sometimes the antibody will bind other targets not intended for visualization and make it difficult to locate the target.

3) Ability of the antibody to diffuse to the target. Sometimes the target protein it sequestered in a location that is difficult to reach with the antibody.

Answer 3

Its disadvantages are as follows:

1. Its use is still limited to analysis of larger cells as it is a type of optical microscope.

2. Sometimes the images may appear blurry.

3. Fluorophores used in this technique are suspected to photo bleaching.

Answer 4

Fluorescence microscopy is a light microscopy method wherein a specimen is irradiated at wavelengths that excite fluorochromes. Some of its disadvantages are: it is slow compared to other methods and is confined by the wavelength of light.

Answer 5

An electron microscope can magnify millions of times while the best light microscopes can accurately only magnify 1000x. The problem with electron microscopes is that they can only view dead organisms.

Answer 6

=when compared with light microscope electron microscope has grater resolution power so this can be used to study ultra structure of the cells and cellulr components.== where light microscope has limited use and only provides the morphological view of a cell=

Answer 7

One advantage of light microscopy over electron microscopy is its relative cheapness. Another advantage is that specimens can be viewed in color using light microscopy while it can only be viewed in black and white using electron microscopy.

Answer 8

Light microscopy is great for small labs and general use, as the microscope is lightweight, usually portable, and relatively inexpensive. For very small particles, however, electron microscopy may be needed. By using electrons instead of light, a much higher resolution can be obtained. Electron microscopes are typicall found in large labs (like universities or corporations) and are quite expensive.

Answer 9

Because only reflected excitatory light is passed through, and transmitted light is filtered out, it creates a much higher intensity. Image clarity is also higher.

Answer 10

light microscopy does not kill the specimen (TEM and SEM does), allowing study of living cells