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Advantages and disadvantages of uv spectroscopy?
Advantage: non-destructive to the sample
Disadvantage: High detection limit
Disadvantage: High detection limit
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Ultraviolet (UV) spectroscopy is a method of determining which wavelengths (colours) of visible light a sample absorbs or emits. There are three main types of UV spectroscopy:… transmittance, absorbance, and emission. Transmittance and absorbance spectroscopy are opposites of one another. In transmittance spectroscopy, different wavelengths of monochromatic light are shot at a sample, and the wavelengths that do not interact with the sample are measured by a detector on the other side of the sample. In absorbance spectroscopy, the light that is absorbed (that is to say, the light that does interact with the sample) is measured using the same setup. If one were to take a graph of transmittance and invert it, it would be an absorbance graph. Emission is done in a slightly different way. Often, when molecules absorb light, they are put into a higher energy level (in one way or another, rotationally, vibrationally, electronically, etc.) which is less stable than their ground state. When the molecules returns to its ground state, it releases light of equal wavelength to that it absorbed. The prime difference between the light that is emitted and the light that was absorbed is direction. Emitted light can be released in any direction. Thus, in order to avoid measuring light from the source, rather than only light that has been emitted, detectors in emission spectroscopy are often put at a 90 degree angle from the source light beam.
Some disadvantages to spectroscopy are that it is only effective at low analyte concentrations, it can be greatly influenced by stray light radiation, and that absorbance must… be taken near the peak absorbance otherwise the value may vary greatly.
From my understanding of this method of analysis,I believe the advantage of AAS lies in the fact that it can can be used to detect the presence of metals singly that is indivi…dually but its disadvantage is that it is an expensive method of analysis since one has to purchase different hollow cathode lamps for different metals. Additional advantages of AAS, include; 1. Greater sensitivity and detection limits than other methods 2. Direct analysis of some types of liquid samples 3. Low spectral interference 4. Very small sample size
effect of solvent on UV-Visible spectrum
UV Visible spectroscopy measures the response of a sample to ultraviolet and visible range of electromagnetic radiations. Molecules and atoms have electronic transitions while… most of the solids have interband transitions in the UV and Visible range. Most molecules have a pi to pi* transition, involving pi electrons. The most important kind of UV/Vis Spectr. is Dispersion based spectroscopy. It involves a disperive medium like prism or grating to separate the different wavelengths.
Differences are: First: There different Wavelength-Range: UV: 200 - ~400 nm; Vis: ~400 nm - ~800 nm.Second: In the Molecules there are different Parts/Effects cause for t…he Absorption in this Regions. For a visual Explanation you may visit the 'Wavelength ~ Energy Diagram' in the related Links below. - (There is also a printable Version.)Third: For UV you need Quarz-Cuvettes.Fourth: 'Nothing else'.
analysis of the transition metals and highly conjugated organic compounds
The richness of spectral features enhances the probability of overlapping absorption bands.The narrow path-length cells required for many analysis are inconvenient to use and …may lead to significant uncertainties.
UV visible spectroscopy is very useful in analyzing dyes and pigments. It may be also used to do kinetics on dye hydrolysis.
UV-Vis refers to ultraviolet-visible spectroscopy, where UV-Vis measures absorption or reflectance in the ultraviolet-visible spectral range. Using light in the visible and ad…jacent ranges, such as UV and IR, excites molecules to undergo electronic transitions. UV-Vis measures ground state to excited state transitions.
actually captopril is a PH sensative drug, as per IP-in 0.1N HCL it's showing 212nm, and it's very difficult to find out lambda by UV-spectroscopy because the UV visible range… of UV- is 200-400nm, and generally near to 200nm all lambda consider as solvent pick....so UV-spectoscopy is not perfact one for analysis purpose of captopril.
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The disadvantage are that it can cause skin cancer if you are exposed to sufficient amounts of UV light (in particular, UV-B and UV-C light). See the Related Questions link to… the left of this answer for more information about that: "How do UV-B rays affect people?" One of the beneficial uses of UV light are that it can be used to kill bacteria and other microbes. It is commonly used to purify water for instance or clean things. UV light can also be used for a variety of other of application, such as black lights (which just look cool!). Also many compounds fluoresce (they emit light) when exposed to UV light. This is how forensic scientists can find traces of blood and other bodily fluids at crime scenes for instance. Finally exposure to some UV-B light causes the body to make Vitamin D, an important nutrient. While some exposure is important too much will cause sunburns and possibly cancer.
difference between uv and visible spectroscopy
the solvent must have high transmittance, i.e, low absorbance.