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What advantage does the streak plate method have over the pour plate method?
Wiki User
∙ 12y ago
A disadvantage of the streak plate technique could be colony isolation problems. If the streaking technique is not done properly or if there is too much of an organism present on the inoculating loop then the cells can cluster and form what looks like one colony but it can actually be a couple colonies (made from a couple cells). This can cause an inaccurate colony forming unit count.
Wiki User
∙ 13y ago
Wiki User
∙ 13y agoStreak Plate method cannot be used for the enumeration of the approximate number of bacteria in the given sample. It can used only for obtaining isolated colonies in Pure culture. This is because, In streak plate method,
1. the amount of inoculum added is not a measured quantity.
2. colony count method cannot be applied on streak plate, since except in the fourth quadrant, isolated colonies are not formed.
Thus Streak plate method can be used for qualitative and not quantitative studies, which is its disadvantage.
Wiki User
∙ 12y agoIt is more likely to give individual colonies regardless of the concentration of the original source. With pour plates, you might have to use several plates with different dilutions of inoculum to get individual colonies.
Wiki User
∙ 15y agoIn streak plate method we can isolate the different types of bacteria from the given suspension, but we can not count the total number of bacteria present in that suspension
Wiki User
∙ 13y agoSimple stains cause all cells in a smear to appear more or less the same color regardless of the type of cells.
Wiki User
∙ 13y agoso that we can dilute the consentrated bacteria (or whatever) into forming a single colony that can be idntifiied with the naked eye
Wiki User
∙ 13y agoStreak plating allows for the formation of individual colony forming units for isolation by reducing the number of bacterial cells with each streaked quadrant.
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What is the criteria for a good quality streak plate?
In scientific circles, the streak plate method is considered to be a rapid qualitative isolation method. To be effective, one must reduce the number of organisms in the inoculums by spreading a loop of culture over an agar plate. This ensures that individual cells are properly separated on the surface for the purpose of differentiating various species. The method is as follows: Using a sterile loop, microbes are initially transferred to the plate with one swipe. On the subsequent swipes, the loop is heated in the flame of a Bunsen burner to lessen the population of microbes being transmitted. Streak patterns are also done in via T-streak or by applying the loop to four quadrants of the plate.
Why does the streaking method you used to inoculate you plates result in isolated colonies?
In the streak plate technique, a loop is used to streak the mixed sample many times over the surface of a solid culture medium in a Petri plate. Theoretically, the process of streaking the loop repeatedly over the agar surface causes the bacteria to fall off the loop one by one and ultimately to be distributed over the agar surface, where each cell develops into a colony.
What is the main advantage of this new method of pancreatic cell transplant over other pancreatic transplant method?
Only half of the pancreas needs to be replaced
How could you identify a potential contaminant on a streak plate?
To clean a streak plate I use coke classic. In the red can, the diet version does not work as well. I believe it is the acid that takes away the powdered rock debris. It makes them quite white again. Mr clean magic eraser also takes away some of the heavy debris...but does not get them clean enough.
What are the Different methods used to isolate microorganisms?
1 The Spread Plate: If a mixture of cells is spread out on an agar surface so that every cell grows into a completely separate colony, a macroscopically visible growth or cluster of microorganisms on a solid medium, each colony represents a pure culture. The spread plate is an easy, direct way of achieving this 2 The Pour Plate: Extensively used with bacteria and fungi, a pour plate also can yield isolated colonies. The original sample is diluted several times to reduce the microbial population sufficiently to obtain separate colonies when plating result. 3 The streak plate: Pure colonies also can be obtained from streak plates. The microbial mixture is transferred to the edge of an agar plate with an inoculating loop or swab and then streaked out over the surface in several patterns
What advantages does the streak-plate method have over the pour plate method?
The streak plate method makes it easier for colonies of bacteria to grow. It also generally leads to individual colonies that look like small dots, rather then simply a mat of bacterial growth.
What advantages does the pour plate method have over streak plate method?
The streak plate method makes it easier for colonies of bacteria to grow. It also generally leads to individual colonies that look like small dots, rather then simply a mat of bacterial growth.
What does the streak plate method have over the pour plate method?
It is more likely to give individual colonies regardless of the concentration of the original source. With pour plates, you might have to use several plates with different dilutions of inoculum to get individual colonies.
What is the criteria for a good quality streak plate?
In scientific circles, the streak plate method is considered to be a rapid qualitative isolation method. To be effective, one must reduce the number of organisms in the inoculums by spreading a loop of culture over an agar plate. This ensures that individual cells are properly separated on the surface for the purpose of differentiating various species. The method is as follows: Using a sterile loop, microbes are initially transferred to the plate with one swipe. On the subsequent swipes, the loop is heated in the flame of a Bunsen burner to lessen the population of microbes being transmitted. Streak patterns are also done in via T-streak or by applying the loop to four quadrants of the plate.
What is the best method for finding the streak of a mineral?
Rub a finger over the mineral
What is the advantage of wet method over dry method in chemistry analyzer?
It is easier to use
What is the main advantage of pour-plate method over other methods of bacterial colony isolation?
Previous answer: "because of infection" This person is obviously trying to be funny, the right word should be "Contamination", as for spread plate, the bacteria is more exposed to air as it is spread over the agar plate. Therefore, the result might not be accurate, as it might be contaminated. As for the pour plate method, the bacteria is in the agar itself, it is not exposed to air, thus, less risk of getting contaminated.
Why does the streaking method you used to inoculate you plates result in isolated colonies?
In the streak plate technique, a loop is used to streak the mixed sample many times over the surface of a solid culture medium in a Petri plate. Theoretically, the process of streaking the loop repeatedly over the agar surface causes the bacteria to fall off the loop one by one and ultimately to be distributed over the agar surface, where each cell develops into a colony.
Primary purpose of the streak plate?
A streak plate technique is used to isolate a single species from a mixed species population. You take a small sample of the mixed species on a sterile loop and streak an agar medium into four zones, reflaming the loop between zones. After incubation, single species colonies should be visible within the fourth zone.
What are the advantages of a CCD over a photographic plate?
Instantaneous results. The primary advantage of the photographic plate over CCDs is image quality--an 8x10 plate carries a lot more image information than a CCD can capture.
What is the function of hockey stick in spread plate method?
The hockey stick spreads the known concentration of bacteria evenly over the agar plate.
What is the main advantage of this new method of pancreatic cell transplant over other pancreatic transplant method?
Only half of the pancreas needs to be replaced
