0
Coz we can
Wiki User
∙ 6y ago
Add your answer:
Earn +20 pts
Process that restriction fragments of DNA are separated from each other by the use of electricity?
The process is referred to as gel electrophoresis. This is an analytical process where DNA fragments can be separated based on size within a gel under the influence of an electric field
What holds the DNA sample during electrophoresis?
DNA samples are within the gel matrix during electrophoresis. DNA moves at differtent rates through the pores of the gel depending on how long the fragments are. DNA is held by the gel itself.
What is the blue band at the bottom of the gel when PAGE gel is performed and where are the DNA fragments?
The blue band at the bottom of the gel is Bromophenol blue and DNA fragments will be in the gel according to their molecular weight.
What is used to make the DNA visible on the gel?
YES!! You can use a simple Agarose gel to separate to view the DNA on electrophoresis. Use 0.8 - 1% gel for 5-10kbp , 2% for 0.2 - 1kbp. If the fragments are really tiny, use an Acrylamide gel (vertical gel) to electrophorese and they will show right out. This is to offset the instability of high concentration gels.
Where is the dna placed in gel electrophoesis apparatus?
In the well made in gel with gel loading buffer
In gel electrophoresism DNA fragments migrate toward one end of a gel because that are?
DNA is naturally negative therefore when a positive charge in put to one side of the gel the DNA wants to move towards it.
Why is gel electrophoresis important?
To separate and analyze DNA fragments and protein fragments by weight. If you have digested some bacterial DNA, for instance, then you can tell by running the fragmented DNA in the gel whether you have digested the correct base length.
How are DNA fragments separated on DNA fingerprinting?
Gel electrophoresis
Can we use the UV light in laminar air flow to see DNA bands in agarose gels stained with Ethidium bromide?
One cannot use the UV light installed in a laminar air flow hood to visualize DNA in an agarose gel. You will have to use an instrument called a UV transillumunator, which illuminates the gel from below to see the stained DNA.
Where do you get the DNA fragments for Gel electrophoresis?
The DNA fragments comes from the method of DNA isolation.
Is agarose gel electrophoresis suitable for only gram negative plasmid DNA?
Agarose gel electrophoresis is suitable for ALL DNA.
Describe the function of electricity and the agarose gel in electrophoresis?
The electricity pulls the polar DNA strands through the gel, and shorter DNA strands move farther because they are less inhibited by the gel. The gel acts as drag to separate the different length DNA strands, so different DNA creates specific dye bands.
