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How can you differentiate gram positive and gram negative bacteria?
Wiki User
∙ 12y ago
The process of gram staining is simple.
1)smear bacteria from pure culture onto slide, heat fix
2)flood with crystal violet (1min)
3)Add iodine (1 min)
4)acid/alcohol wash (1 min)
5)Flood with safranine (1min)
6)Air dry and examine.
These times are for clinical microbiology and experimental methods employ optimal and more precise times (but overall its pretty close).
Down side of this method is that you must smear bacteria onto the slide and fix it by heating the underside of the slide with a bunsen burner. if they are pink then you have gram negative (Gram's stain didnt stick) if its purple then its gram positive(Gram's stain did stick) This is due to the peptidoglycan layers. Gram negative bacteria have only a thin layer of peptidoglycan as part of the cell membrane/wall where Gram positive have a very think peptidoglycan layer.
Source(s):
Medical Microbiology
Wiki User
∙ 12y ago
Wiki User
∙ 13y agoNormally gram positive bacterium have a thick peptidoglycon layer where as gram negative bacterium has a thin peptidoglycon layer . The lipopolysaccharide content is more in gram negative bacteria but its not present in gram positive . There is a staining procedure called gram staining which differentiates between a gram positive from gram negative bacteria . In this staining procedure the bacteria is treated with crystal violet , iodine , ethanol and safranin in which the gram positive does not take pink colour of safranin and gram negative appears pink. outer membrane is present in negative not in positive .
Wiki User
∙ 16y agosearch Gram's stain - its the No1 differential stain.
Wiki User
∙ 13y agomainly by the color differentation we can indentify the organism, generally the gram positive bacteria appears in purple in colour and gram negative bacteria appears pink in colour
Wiki User
∙ 12y agoLab test will tell.
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Is anthrax gram positive or negative?
gram positive bacteria
What is most common gram positive or gram negative bacteria?
gram negative
What colors gram positive and gram negative bacteria?
Gram-positive cells are purple and the Gram-negative cells are red.
Are archaebacteria gram positive or gram negative?
A guess ;positive or negative bacteria spider(insect).
How Can you elimintate gram positive bacteria from a gram positive gram negative mixture with mild detergent treatment?
as gram negative have low content of phospholipids than positive detergents at low concentration effectively kill negative bacteria
Is klebsiella gram positive or negative?
i believe it is a gram bacteria which causes respiratory illness.
What is the advantage of the gram stain over the simple stain?
gram staining differentiates between gram negative and gram positive bacteria by showing different colors. it shows blue or purple like color for gram positive bacteria and red color for gram negative bacteria. where as simple stain gives the same color to all the types of bacteria. hence it is difficult to differentiate between them.
Is anthrax gram positive or negative?
gram positive bacteria
Name the different gram positive and gram negative bacteria?
Name of gram positive and gram negative bacteria?
Does Tetracycline kill gram positive or gram negative bacteria?
Tetracycline kills both gram positive and gram negative bacteria. The mode of action to which Tetracycline works to kill bacteria is that it inhibits protein synthesis which works against both gram positive and gram negative bacteria.
What is most common gram positive or gram negative bacteria?
gram negative
Is lactobacillus casei is a gram negative bacteria or gram positive?
The Bacteria Sarcina lutea is Gram Positive Because If You Perform the Gram Test It Will Turn The Violet Color Which indicates It's Positiveness. What Makes It Positive Is the Peptidoglycan Located Within The The Bacterium
Is gram positive bigger than gram negative?
Gram-positive bacteria and Gram-negative bacteria are different in their colouration when dyed and viewed with a light microscope. Gram-positive appear violet, and Gram-negative appear red. Gram-positive and Gram-negative classification, however, has nothing to do with size.
What colors gram positive and gram negative bacteria?
Gram-positive cells are purple and the Gram-negative cells are red.
What is the Bacteria in gingivitis is it gram positive or negative?
gram +
Are archaebacteria gram positive or gram negative?
A guess ;positive or negative bacteria spider(insect).
Which Gran Positive or negative which is more serious?
Gram positive bacteria responds to the Gram stain; gram negative bacteria does not. The two bacteria do not respond to the same antibiotics. Right now the most dangerous bacteria is a gram negative bacteria. That could change.
