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How can you estimate the size of the genomic library you want and need to develop to be sure that a particular gene is present in that library?
Wiki User
∙ 12y ago
probability of including any DNA sequence in a random libray of N independent recombinants:
N= [ln(1-P)] / [ln(1-1/n)]
where n= genome size /average insert size
in theory, a genomic library should contain all the different sequences,
but in practice, a genomic libray is never perfect.
Wiki User
∙ 12y ago
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What is the complete collection of cloned DNA fragments from one organism?
it is called genomic library
A set of bacterial clones collectively have fragments representing all of the DNA from another organism in them is called a?
genomic library, a set clones that collectively contain all of the DNA in an organis's genome
What is the use of DNA library?
cDNA is the short form complementary DNA. cDNA libraries are a combination of cloned cDNA fragments. cDNA libraries are used to express eukaryotic genes in prokaryotes.
What is the definition of genomic footprinting?
Same as genetic footprinting, only at a high throughput, systems level way. Typically you use a transposon library to insert mutations to cells that inactivate genes (you assume one transposon/inactivation per cell). Then you trascription profile (dna microarray, rna-seq) after selection (whatever you want to select for, growth, resistance etc) and try to map the mutations/genes inactivations to the fitness changes in the cells. For example if you select for growth in a particular environment and the deletion of a gene confers this, then during selection that mutant will be over-represented, so when you run a microarray, you will see no expression of the deleted gene in reference with the wild type strain.
What are plasmid libraries?
A plasmid is a piece of circular DNA. These plasmids are transformed into bacteria/yeast. A library is made by taking pieces of DNA (from a genomic DNA digest, or cDNA) and inserting them into plasmids. The plasmids are then transformed into the organism and stored. Libraries are used to screen for new functional genes, usually when looking at new substrates or products that the yeast/bacteria can't make without the plasmid.
How is a genomic library produced?
A genomic library is produced through the meticulous accumulation of genetic information that lends to different classifications and structures to the level of taxonomy.
What is use of Genomic library?
cloned genes -sarahhh(:
Difference between genomic library and cdna library?
A cDNA (complementary DNA) library is a DNA library that has been created from mRNAs that are present in the cell. Since a cDNA is created from mRNA transcripts, that means that in Eukaryotic organisms there will be no introns or transcriptional factors present in the cDNA library, only exons. Only protein coding regions will be present in a cDNA library. This also means that a cDNA library is often times tissue specific. Since the expression of mRNAs will be different in different tissues of the organism it will appear different then a genomic library. Often times to offset this problem a cDNA library will be composed of different tissues (brain, liver, heart) to encompass a greater variety of the proteins that are expressed. A genomic library will contain all the exons, introns, and transcriptional factors that are not found in the cDNA library. **2/24/2011** cDNA library does contain exons, which is the protein coding regions.
What is the complete collection of cloned DNA fragments from one organism?
it is called genomic library
The DNA fragments making up a genomic library are generally contained where?
Recombinant plasmids of bacteria
What is the most important consideration for genomic library?
lirrary it is the work as a one type of referances for our purposes
A collection of cloned recombinant plasmids that includes fragments from the entire genome of a cell is called a?
Genomic library
What has the author Bennet Deakin written?
Bennet Deakin has written: 'Inter-generic genomic library transfer into Arabidopsis thaliana identifies a NAC gene involved in cell expansion'
A set of bacterial clones collectively have fragments representing all of the DNA from another organism in them is called a?
genomic library, a set clones that collectively contain all of the DNA in an organis's genome
What is special in special library?
special library is a library which caters to a particular kind of users, specific kind of subject and specific kind of material.
What is a genomic library?
Specific human genes stored in virus, bacteria and yeast hosts no, the genes are randomly inserted into vectors. a cDNA library houses tissue-specific sequences derived from an mRNA transcript so that it contains only genes that code for protein.
How do you use particular in sentence?
Do you fancy any particular restaurant? I'm off to the library as I'm looking for a particular book. I dress up for church on Sundays as I'm very particular on how I look.
