0
- Check for any non-granular sediments if the broth was stationary.
- Check for turbidity in the broth.
Turbidity can be checked by tilting the flask towards light and slowly rotating it while keeping an eye on any difference in light transmittance/color/texture/threads/anything that is different from the rest of the broth!
If not sure, autoclave it again!!
P.S. Turbidity and sediments can only be visible if there was ample incubation, it will NOT be visible at the moment you make it!!
Other advanced way can be in the use of spectroscopy. Take fresh broth as blank and suspected broth as sample and check for optical densities. If the suspect one has a greater turbidity than the fresh one then probably your broth is contaminated.
P.S. Right wavelength is the key! It should be set according to the color of your broth!
Good luck!
Wiki User
∙ 12y ago
Wiki User
∙ 15y agoIf you autoclave it for 15 mins at 121oC and at 15lbps pressure then it is called sterlized and to check it just put it in incubator with shaker for 24 hrs it got turbidit due to microbial cells growth then it is said to be not sterile and if there is no cell growth in it then it is sterile
Wiki User
∙ 14y agoYou go and get tested.
Add your answer:
Earn +20 pts
Can you determine whether a agar deep culture is pure by visually inspecting it without a microscope?
The short answer is no. Microorganisms are just that - microscopic, and the naked eye can not determine between different species, especially in a broth culture. Maybe on a plate, where the colony shapes, sizes, viscosity, and color can be determined, but a broth culture usually needs to be streaked onto a plate to determine if it is pure or not.
Why is it incorrect to say that an object is partially sterile?
This definition is only one or the other. Either sterile or non-sterile. There is no such thing as partially sterile.
Explain the results in the sterilized and not-sterilized broth?
Sterilized broth is clear, meaning no bacterial growth. Non-sterilized broth is cloudy.
Is mucous membranes sterile?
Are mucous membranes sterile
Should tracheostomy care be sterile or aseptic?
sterile
What is Pasteurs experiment?
Showed that sterile broth remained free of microorganisms until exposed to air.
Who discovered the falsified spontaneous generation?
Pasture - he sealed a boiled broth in a glass container. It stayed sterile.
What would have happened is Pasteur had tipped on of his flasks so that the broth in the flask came into contact with the curve of the neck?
If the pasteur tipped on of his flasks so that the broth came into contact with the curve of the neck, the sterile broth soon became contaminated with microorganisms which were trapped in the curve.
Can you preserve or freeze a bacterial isolate in a normal freezer by just growing them in a broth with glycerol?
Add the sterile glycerol after growing them in broth. However, -80 freezer preserves them for longer than normal freezer.
Are you sterile if you have a negative blood type?
Blood type does not determine sterility.
How does thioglycollate work?
Thioglycollate broth is an enriched medium used to determine the oxygen requirements of microorganisms. The diffusion of oxygen from the top of the broth provides a range of concentrations.
What kind of media must one use to determine chitinolytic activity?
Nutrient broth
Can you determine whether a agar deep culture is pure by visually inspecting it without a microscope?
The short answer is no. Microorganisms are just that - microscopic, and the naked eye can not determine between different species, especially in a broth culture. Maybe on a plate, where the colony shapes, sizes, viscosity, and color can be determined, but a broth culture usually needs to be streaked onto a plate to determine if it is pure or not.
What is the broth in the flask?
In a science experiment, a broth is a liquid and a flask is a particular shape of glassware. If the method of your experiment refers to "the broth in the flask", look back over the previous steps and figure out what chemicals and liquids you added to the flask. If the goal of the experiment is to determine what the unknown broth in the flask is you may have to determine it by observing it's physical and chemical traits, be careful with the unknown broth in case it is flammable, poisonous or otherwise dangerous.
Can a dr determine how long you've been sterile?
Yes! Ask your Gynecologist! Good luck!
How can you tell if he is sterile?
The only way to determine if a man or woman is sterile is to have them tested. There are a series of tests that can be done on a sample of the man's sperm to determine if he is sterile. As a rule of thumb this is what a man needs to be considered fertile: 2ml or more of semen 2 million sperm per ml of semen 50% or more of the sperm must be correctly formed 50% or more of the sperm must have good motility
Is a flower sterile or non sterile?
the flower is sterile
