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DNA has a double helix structure. This double helix structure exists, as the bases form hydrogen bonds (Adenine-Thymine form 2 hydrogen bonds and Guanine-Cytosine form 3 hydrogen bonds). In an alkali environment (mostly NaOH is used) the hydrogen bonds between the bases will be broken by the hydroxylic group (OH). Due to the breakage of the hydrogen bond, the double helix is broken, so two single DNA strands are obtained. Meaning the DNA is denatured.
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Neutral solutions are neither .............?
Acid and Alkali.
Does boiling denature cells?
Boiling can allow for the extraction of DNA.
What is the name of a no acid and alkali substance?
They are called as neutral solutions. A common example is pure water.
What metals that lose one electron when they react with water to form alkaline solutions?
Alkali metals
What does alkali labile site mean?
a weakened polymer structure at the site of the attack of DNA
What can denature DNA?
by heating above certain temprature eg.90 or 100 degree celcius or by treting with strong alkali or strong acid you can denature your DNA *Actually, you can denature DNA in water if you wanted to. Basically any polar solvent will denature DNA because it has a negatively charged sugar-phosphate backbone. Mutagens can also influence DNA although it isn't exactly denaturing it. So can high energy light, like UV or all kinds of radiation. This, too, isn't denaturing though.
What is the function of formamide loading buffer?
To denature DNA
Why must DNA fragments be exposed to an alkaline solution before hybridization?
To denature the DNA
How does formamide denature DNA?
Through the magic of hydrogen bonding
What are common alkali solutions?
Soaps
Neutral solutions are neither .............?
Acid and Alkali.
Why is cold ethanol better than room temperaure for DNA isolation?
DNA does not dissolve on Alcohols. It is better to store it in cold temp. for it not to denature
What is the role chelex-100 in DNA extraction?
Chelex is there to simply denature the Double stranded DNA and convert it to single strand DNA so the primers can attatch to it during PCR.
Does boiling denature cells?
Boiling can allow for the extraction of DNA.
What is the first step in the polymerase chain reaction?
It allows the biologists to make many copies of a dna strand...
Can you tamper with DNA?
Not directly. Radiation can cause mutations in DNA. Excess heat (as in the case of a fever) can denature (destroy) the DNA sequence as well as other proteins which will usually result in cell death.
Is sodium alkali or acid?
The water solutions of sodium chloride are neutral.
