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If you are using a spectrophotometer to read the samples then you take the tube with the greatest amount of haemolysis as the 100% tube.
Then you place the absorbance readings of the other tubes over the absorbance reading in the 100% tube and multiply by 100.
E.g 100% = Abs of 1.302
Unknown = Abs of 0.620
0.620/1.302 x 100 = % Haemolysis in that tube.
Hope that helps :P
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