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it can't be heated to inactivation,instead you can just try protease K treatment

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Q: How do you inactivate MspI restriction enzyme?
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An enzyme that cuts double-stranded DNA at specific nucleotide sequences?

Such an enzyme is called a restriction endonuclease


What is the restriction site of the restriction enzyme TaqI?

TaqI's restriction site is:TCGAAGCT


Hi does any one know if I want to put some restriction enzyme close to cDNA should I put some nucleotide befor this restriction enzyme?

Restriction enzyme cuts DNA strand at specific locations Restriction enzyme cuts DNA strand at specific locations


Restriction enzyme digestion?

Yes?


How do you identify restriction enzyme?

By playing with you.


Is topoisomerases belong to restriction enzymes?

Topoisomerase is not a restriction enzyme but an enzyme that keeps unwound DNA from tangling while it is being replicated.


Why restriction enzyme cannot cut its own DNA?

Restriction enzymes are produced by bacteria to help destroy foreign, invading DNA, such as the DNA of bacteriophage (a virus that infects bacterial cells). Every restriction enzyme comes with a methylase enzyme, or more specifically, a DNA methyltransferase. The methylase enzyme methylates (adds a methyl group) to the restriction endonuclease site on the cell's own DNA, which protects the sites from the restriction enzyme so that it does not degrade its own DNA.


Why did you cut both segments of DNA with the same restriction enzyme?

You use the same enzyme inn order to get the same restriction and binding sites.


What type of cbrne agent acts by inhibiting a cruicial enzyme called acetylcholinesterase?

Nerve agents are compounds that have the capacity to inactivate the enzyme acetylcholinesterase (AChE)


Which enzyme is used to produce RFLPs?

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How does enzyme restriction limit the affect of a virus?

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