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How does DNA technology transfer bacterial genes from one cell to another?
Wiki User
∙ 14y ago
Gene transfer between cells generally consists of the following steps:
1. Isolating the gene of interest - Here, the gene which has to be transferred has to be isolated from the genome of the source (or host) organism.
2. Splicing the gene if interest into a plasmid. Splicing is a process wherein a foreign strand of DNA (the gene of interest) is inserted into a loop of DNA called a plasmid. The plasmid DNA is cut open to form a linear fragment. The gene of interest is then attached to the plasmid DNA. The plasmid DNA is converted back into the loop form with the help of an enzyme called DNA ligase.
3. Gene amplification: Here, the plasmid containing the gene of interest is amplified. Which means, many copies of the plasmid containing DNA are created through a process called the polymerase chain reaction.
4. Transfection: This is the final step wherein the plasmid containing DNA is inserted into the recipient organism. Sometimes the foreign DNA remains within the plasmid and is able to express protein. Other times, the gene of interest can be engineered to contain a sequence called the recombination sequence which will allow it to integrate (or join) the host genome through a process called homologous recombination.
By the method described above, a foreign gene is removed from one organism and inserted into another. If the gene of interest is integrated into the host of the recipient organism, copies of it are made every time the host cells divide.
Wiki User
∙ 14y ago
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Which substance is responsible for transfer of characteristics from one generation to another?
The genes found in DNA transfer characteristics from one generation to the next.
How are genes moved in and out of the gene pool?
Gene Flow and Genetic DriftGene Flow- transfer of genes from one population to another of the same speciesGenetic Drift- a change in the pool of genes of a small population that takes place by chanceAnd another possible answer could be breeding/mating
What is the difference between lateral and vertical gene transfer?
Lateral gene transfer is the process of genetic transfer of (mostly) resistance genes through plasmids among bacteria while vertical gene transfer is the transfer of genetic materials that are passed down from parent organism to offspring from one generation to another.
What plasmids are and how they are used to get bacteria to synthesize a new protein that they normally do not synthesize?
Plasmids are circles of DNA. These have genes that can be transferred from one bacteria that has it to another. These genes can code for a protein that one cell normally doesn't code for. This done by a process called bacterial conjugation.
What is an extrachromosomal piece of bacterial DNA that contains genes not essential for cell growth?
A Plasmid
How does DNA technology transfer bacteria genes from cell to cell?
DNA technology will transfer bacteria genes from cell to cell.
The transfer of genes during bacterial conjugation involves rigid tubular appendages called what?
They are called sex pili.
Which substance is responsible for transfer of characteristics from one generation to another?
The genes found in DNA transfer characteristics from one generation to the next.
Who transfer the biological informantion genes or gamets?
The genes transfer the biological information. If you mean passing genes on to the next generation, it would be the genes in the gametes.
What is mammalian cloning?
its a type of experiment that uses the original genes of a mammal and transfer it to another mammal
How can specialized transduction contribute to the transfer of antibiotic resistance genes in a bacterial population?
The prophage takes an antibiotic resistance gene with it and is packaged with the newly synthesized viral DNA.
Explain how DNA technology transfer bacterial genes from cell to cell?
I think you must rethink about your question, but still I am giving the answer as I can understand that you are asking about recombinant DNA technology where bacterial DNA is used as it is a cloning vector (plasmid). In recombinant DNA technology the particular sequence of DNA that we want to replicate or want to produce in huge number, is attached either with plasmid of bacteria or a DNA of bacteriophage and thus produce the recombinant or hybrid DNA which is copied each time when the bacteria or bacteriophage multiply. In this way the hybrid DNA will be transferred from parent cell to daughter cells.
How are genes moved in and out of the gene pool?
Gene Flow and Genetic DriftGene Flow- transfer of genes from one population to another of the same speciesGenetic Drift- a change in the pool of genes of a small population that takes place by chanceAnd another possible answer could be breeding/mating
Which genes can be transferred by all three methods of intermicrobial transfer?
The genes that can be transferred by all three methods of intermicrobial transfer are drug resistance. Horizontal transfer is when the individual cell pass along the resistance genes.
In what part of a bacterial cell would you find the genes?
cytoplasm
What is gene flow?
Gene flow is genes moving between two populations. the transfer of genes from one gene pool to another two populations transferring genes
What role does technology play in genetically modified food?
Without technology, there would be no genetically modified foods, because the technology to remove a gene or genes from one species and insert it into another to produce a desired trait would not be available.
