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I can't tell you how it is done in a high-tech lab, but this low-tech way will work!

1. Since we are dealing with a plant cell (such as peas), the cell walls need to be broken. This is done by adding water to the peas (or whatever you are using), and blending them for a few seconds.

2. Add a small amount of Dawn dishwashing liquid (breaks the cell and nuclear membranes).

3. Add a pinch of meat tenderizer (separates the proteins from the DNA).

4. Stir gently.

5. Put about an inch of the material into a test tube.

6. Carefully add about the same amount of rubbing alcohol so that the alcohol forms a layer on top of the "pea soup."

7. Since DNA is less dense than the "pea soup," it will rise and it enters the alcohol layer and appears as a white stringy material.

Try googling "how to extract DNA from anything living."

DL

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13y ago
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14y ago

First, plants have cell walls that make it mechanically more difficult to break open the cells. Secondly, plants have a variety of molecules that can bind to DNA (polysaccharides, polyphenolics, proteins, etc.) making it sometimes very difficult to obtain a relatively pure sample.

Thirdly, like any cell, plants have enzymes which will degrade DNA once they are released by the process of opening the cells.

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14y ago

1) take plant

2) obtain microscope 150X or greater

3) grasp a .000015 M DNA extractor vacuum tube

4) insert tip into mitochondria wall

5) extract DNA substance

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11y ago

Because, Plant cells are harder to extract DNA from, because it has a Cell Wall. Animal Cells are much easier to obtain.

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11y ago

Most plant DNA is found in the same place as any other eukaryote: inside the nucleus. There's also some DNA inside mitochondria and chloroplasts.

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7y ago

A blender is used and that will break down the cell walls of the plant.

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10y ago

the dna does it job ha ha

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Q: How is the cell wall of plant cell broken down during DNA extraction?
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the purpose of grinding any substance during dna extraction is cell loosening.


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