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The first step is restriction of the cosmid and the foreign DNA with the restriction enzyme, then ligating the fragments together. Thereafter, the cosmids are loaded into the phage capsid, which leads to the expression of the foreign gene through transduction.
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What is a small double stranded DNA that's used as a vector?
it is a ds DNA use in recombinant DNA technology to insert our interested gene and multiply it.Ex;plasmid,cosmid
What is a Self replicating DNA used to transmit a gene from one organism to another?
Self replicating DNA used to transmit a gene from one organism to another is a vector. A population of cells carrying a desired plasmid is called a clone.
Why is cloning an example of cloning?
Gene Cloning is used to clone a gene of interest in a vector called plasmid. The chimeric DNA or rDNA formed by cloning is stable and can be used to propagate and sequence the DNA. producing vector containing inulin gene is an example.
What are phage vectors?
"Vector" is an agent that can carry a DNA fragment into a host cell. If it is used for reproducing the DNA fragment, it is called a "cloning vector". If it is used for expressing certain gene in the DNA fragment, it is called an "expression vector".Commonly used vectors include plasmid, Lambda phage, cosmid and yeast artificial chromosome (YAC).
What is the role of Cloning Vector in recombinant DNA technology?
The desired gene is cut from its original DNA by using restriction enzyme. The vector (plasmid) is also cut with the same restriction enzyme so that both the vector and the desired gene have the same sticky end. They are bonded together by DNA ligase. Why desired gene are cloned into vector? This is because vector have the ability to reproduce by itself and at the same time carry the desired gene together; in another word, it can replicated the desired gene in the host cell.
Significance of vector in DNA recombinant technology?
Vector are plasmid DNA, act as a molecular vehicles to carry genes or DNA of interest. In rDNA technology vectors used to clone the gene by ligation. This chimeric DNA or plasmid can be propagated in E.coli as the vector carries its own origin of replication. Expression plasmid vectors can be used to produce proteins from the gene of interest.
In the process of human gene cloning using recombinant plasmids what is the bacterial plasmid?
functions as a vector
Why is it important to isolate and clone diseased gene?
Scientist must isolate and clone diseased genes in order to study them. A clone of the gene allows scientist to find an exact cure.
If you place gene in reverse orientation how can it be detected?
Most genes contain restrictions sites. Once you've inserted your gene into the vector you use restrictions sites in the gene and the vector to cut the vector into smaller pieces. If the pieces correspond to the pattern you expect for a reverse orientated gene then you know it is in the reverse orientation.
How do you predict a PCR product size from a gene cloned into an expression vector?
It all depends on where you primers are. Presumably you will have one primer that sits on the cloned gene and one that sits on the vector (that way you only get a product if the gene has cloned successfully). As long as you know where your primers land, it should be easy to work out how big the PCR product will be simply by adding the distance from the primer on the gene to the end of the gene and the distance from the primer on the vector to the end of the vector.
How can a virus conduct genetic engineering?
A virus can conduct genetic engineering by using it to insert a gene into a cell. The virus containing the gene is called a vector. By changing the cell, you can introduce to other cells.
How do bacteria clone?
bacteria regenerates by cloning, their behaviour will be same and will become smaller, grow again, and again clones, it happens by... in a microscope it will look like a bactiria is been cut into two and will look smaller.
