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It depends on the power of the microscope an the skill you have in preparing slides.

Around 150x, good slide prepartion becomes crucial.

a quick search on Google reveals many guides to building your own Scanning Tunneling Microscope (STM). If you do attempt such a feat the following books might be helpful;

  1. Lewis et al. "Student Scanning Tunneling Microscope" Am. J. Phys, 59(1), 38-42. (1991)
  2. Pohl, "Some Design Criteria in Scanning Tunneling Microscopy", IBM J. Res. Dev., 30(4), 417-427, (1986)
  3. Binning & Rohrer, "Scanning Tunneling Microscopy" IBM J. Res. Dev., 30(4), 355-369, (1986)
  4. Binning & Smith, "Single-Tube Three-Dimensional Scanner for Scanning Tunneling Microscopy", Rev. Sci. Instrum. 57(8), 1688-1689, (1986)
  5. Sears et al., "A Scanning Tunneling Microscope for Undergraduate Laboratories", Computers in Physics Jul/Aug, 427-430, (1990)

I believe that a home made STM can be made for about $100 and should provide atomic resolution.

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Q: If you buy a microscope for the home what level of detail would one be able to see?
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What type of microscope would you need to see detailed images of an internal structure of a bacterium a light microscope or an electron microscope?

Bacteria are generally very small - so you would need an electron microscope to see them in any detail. Under a light microscope you would probably only be able to see the overall shape.


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When viewing objects under - power you are able to see a larger field of view but not as much detail?

Just like when you are closer to an object you are able to see detail, but when you move farther away from it, you see more of the object but less of the detail. For eg, lets consider an example of a calendar hanging on the wall. When you are close to it, you may just be able to see the calender, with the month and the dates and days. As you move farther away from it, you would slowly not be able to see the dates and days too clearly, but you would also be able to see more of the wall on which the calender is hanging. Hence when you are able to see a larger field of view, you see much lesser detail. In scientific terms, the magnification is smaller. As you increase the microscope magnification, you would be able to see more detail, but lesser field.


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How would you able to distinguish parts of the organism using the microscope?

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