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Restriction enzymes (also known as restriction endonucleases) are proteins which cut DNA up at specific sequences in the genome. For example, the commonly used restriction end…onuclease EcoRI recognizes every point in DNA with the sequence GAATTC, and cuts at the point between the Guanine and Adenine. Interestingly, the recognition sequences for most restriction endonucleases are genetic palindromes, e.g., the sequence reads exactly the same backwards on the complementary strand. In the case of EcoRI, the two complementary DNA strands for the recognition sequence are: 5'--GAATTC ---3' 3'--CTTAAG--5'
A restriction enzyme (also known as restriction endonuclease) is protein which cuts DNA up at specific sequences (called restriction sites) in a genome. For example, the commo…nly used restriction endonuclease EcoRI recognizes every DNA sequence GAATTC and cuts at the point between the guanine and the adenine in that sequence, forming blunt ends (or straight, even ends). Interestingly and coincidentially, the restriction site for most restriction enzymes are genetic palindromes (the sequence reads exactly the same backwards on the complementary strand). In the case of EcoRI, the two complementary DNA strands for the restriction site are: 5'-- GAATTC --3' 3'-- CTTAAG --5' After this DNA sequence is cut, it might look something like this: 5'-- G AATTC --3' 3'-- C TTAAG --5'
Restriction enzyme cuts double or single stranded DNA at a specific recognition nucleotide sequences known as restriction site.
Restriction enzymes are used in lab for cutting DNA sequences at specific sites.The RE used will cut only at its recognized site. expose the base sequence of a DNA fragment.… they are enzymes isolated from bacteria.They are of two types.Restriction endonuclease & restriction exo nuclease.exonucleases remove nucleotides from the ends of the DNA .endonucleases make cuts at specific positions in the DNA
Topoisomerase: are isomerase enzymes that act on the topology of DNA Helicase untwists the double helix and separates the template DNA strands at the replication f…ork. This untwisting causes tighter twisting ahead of the replication fork, and topoisomerase helps relieve this strain
Restriction enzymes are obtained from bacteria. "Sticky ends" are produced as a result of the action of a restriction enzyme.
Restriction enzymes are often sourced from bacteria. They cut DNA at specific sequences and so can be used to create fragments of DNA from whole strands. The characteristic pa…ttern of these fragments for different restriction enzymes is used in genetic finger printing of people and bacteria.
The function of topoisomerase is to unwind the helix of DNA into a regular, ladder-like shape. Topoisomerase is only found in primates. In other organisms, the molecule is cal…led gyrase that unwinds the DNA.
Bacteria for protection against viruses synthesize restriction enzymes which cut only viral without giving any harm to bacterial DNA.
The restriction enzyme cleavage site is a specific site on the DNA strand that has a defined sequence of nucleotides. The restriction enzyme recognizes this sequence, binds to… the DNA and produces a cut. For example, for the restriction enzyme EcoR1, the restriction site is GAATCC. Therefore, whenever EcoR1 finds GAATCC, it binds to the DNA at that location and produces a cut
There are many restriction enzymes found in biological system. Few of them are EcoRI, BamHI, HindIII etc.
TaqI's restriction site is: TCGA AGCT
Restriction enzymes or endonucleases are like cutting enzymes fro DNA These are used to cut nucleotides at particular sites These have imp use in gene cloning,gene mapping,g…ene sequencing then applied techniques such as southern blotting These are extracted from bacteria's