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Is topoisomerases belong to restriction enzymes?
Restriction enzymes are made by the ribosomes in bacteria. For information on which bacteria produce which specific restriction enzymes, some can be found in a table nea…r the bottom of this page: http://en.wikipedia.org/wiki/Restriction_enzymes
Restriction enzymes (also known as restriction endonucleases) are proteins which cut DNA up at specific sequences in the genome. For example, the commonly used restriction end…onuclease EcoRI recognizes every point in DNA with the sequence GAATTC, and cuts at the point between the Guanine and Adenine. Interestingly, the recognition sequences for most restriction endonucleases are genetic palindromes, e.g., the sequence reads exactly the same backwards on the complementary strand. In the case of EcoRI, the two complementary DNA strands for the recognition sequence are: 5'--GAATTC ---3' 3'--CTTAAG--5'
A restriction enzyme (also known as restriction endonuclease) is protein which cuts DNA up at specific sequences (called restriction sites) in a genome. For example, the commo…nly used restriction endonuclease EcoRI recognizes every DNA sequence GAATTC and cuts at the point between the guanine and the adenine in that sequence, forming blunt ends (or straight, even ends). Interestingly and coincidentially, the restriction site for most restriction enzymes are genetic palindromes (the sequence reads exactly the same backwards on the complementary strand). In the case of EcoRI, the two complementary DNA strands for the restriction site are: 5'-- GAATTC --3' 3'-- CTTAAG --5' After this DNA sequence is cut, it might look something like this: 5'-- G AATTC --3' 3'-- C TTAAG --5'
Restriction enzymes are used for genetic engineering. they expose the base sequence of a DNA fragment. The enzymes cut the phosphate backbones of DNA molecules at specific bas…e sequences called recognition sites. Strands of DNA that have been cut with restriction enzymes sometimes have single-stranded tails that readily realign with tails from certain other DNA fragments. This technology allows removing a specific gene from one organism and splicing it into another. Restriction enzymes originally developed in bacteria as a defense against viruses, who inject DNA in bacteria which takes over the cell. The bacteria's restriction enzymes cut up viral DNA before it can take over the cell.
Restriction enzyme cuts double or single stranded DNA at a specific recognition nucleotide sequences known as restriction site.
Restriction enzymes are used in lab for cutting DNA sequences at specific sites.The RE used will cut only at its recognized site. expose the base sequence of a DNA fragment.… they are enzymes isolated from bacteria.They are of two types.Restriction endonuclease & restriction exo nuclease.exonucleases remove nucleotides from the ends of the DNA .endonucleases make cuts at specific positions in the DNA
restriction enzymes cut the DNA at specific sites called restriction sites. These restriction sites are 6 - 8 base pairs long and occur at different frequencies on the DNA
Restriction enzymes are obtained from bacteria. "Sticky ends" are produced as a result of the action of a restriction enzyme.
Restriction enzymes are often sourced from bacteria. They cut DNA at specific sequences and so can be used to create fragments of DNA from whole strands. The characteristic pa…ttern of these fragments for different restriction enzymes is used in genetic finger printing of people and bacteria.
The function of topoisomerase is to unwind the helix of DNA into a regular, ladder-like shape. Topoisomerase is only found in primates. In other organisms, the molecule is cal…led gyrase that unwinds the DNA.
The restriction enzyme cleavage site is a specific site on the DNA strand that has a defined sequence of nucleotides. The restriction enzyme recognizes this sequence, binds to… the DNA and produces a cut. For example, for the restriction enzyme EcoR1, the restriction site is GAATCC. Therefore, whenever EcoR1 finds GAATCC, it binds to the DNA at that location and produces a cut
There main purpose is to cut up viral DNA that invades bacteria. Since they do this at definite sites, depending on which restriction enzyme is used, they can be used in genet…ic recombination exercises. Inserting sections of DNA into bacterial plasmids so that desired products will be made by these bacteria.
Restriction enzymes or endonucleases are like cutting enzymes fro DNA These are used to cut nucleotides at particular sites These have imp use in gene cloning,gene mapping,g…ene sequencing then applied techniques such as southern blotting These are extracted from bacteria's