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Is topoisomerases belong to restriction enzymes?
They race along strands of DNA and RNA looking for a specific sequence where it cuts and renders that DNA harmless. The most common purpose is for security. If a foreign molec…ule from a virus or somewhere else comes into a cell, it is cut up to stop it from being transcribed. When these were discovered, scientists used them for genetic engineering by opening a DNA molecule to insert a gene
The function of topoisomerase is to unwind the helix of DNA into a regular, ladder-like shape. Topoisomerase is only found in primates. In other organisms, the molecule is cal…led gyrase that unwinds the DNA.
Restriction enzyme in bacteria cuts of the foreign DNA inside the host, thus destroying them. The host DNA is protected against this destructive action due to methylation of t…he host DNA.
Restriction enzymes (also known as restriction endonucleases) are proteins which cut DNA up at specific sequences in the genome. For example, the commonly used restriction end…onuclease EcoRI recognizes every point in DNA with the sequence GAATTC, and cuts at the point between the Guanine and Adenine. Interestingly, the recognition sequences for most restriction endonucleases are genetic palindromes, e.g., the sequence reads exactly the same backwards on the complementary strand. In the case of EcoRI, the two complementary DNA strands for the recognition sequence are: 5'--GAATTC ---3' 3'--CTTAAG--5'
A restriction enzyme (also known as restriction endonuclease) is protein which cuts DNA up at specific sequences (called restriction sites) in a genome. For example, the commo…nly used restriction endonuclease EcoRI recognizes every DNA sequence GAATTC and cuts at the point between the guanine and the adenine in that sequence, forming blunt ends (or straight, even ends). Interestingly and coincidentially, the restriction site for most restriction enzymes are genetic palindromes (the sequence reads exactly the same backwards on the complementary strand). In the case of EcoRI, the two complementary DNA strands for the restriction site are: 5'-- GAATTC --3' 3'-- CTTAAG --5' After this DNA sequence is cut, it might look something like this: 5'-- G AATTC --3' 3'-- C TTAAG --5'
those place where restriction enzyme cut the DNA molecules
Restriction enzymes can leave blunt or sticky ends. This will depend on where they cut the DNA, if they cut at the same point on both strands, they will leave a blunt end, if …they cut at different points, they will leave sticky ends. For example: (| represents point of cut) Blunt end ATC|GCTA TAG|CGAT Sticky end A|TCGCTA TAGCGA|T
They are the bacteria's immune system designed to cut up and destroy invading bacteriophage ( Viruses that attack specific bacteria)They recognize specific sequences of DNA fo…und in the virus and cuts them, destroying the virus' capability to survive and reproduce
Restriction enzymes protect the DNA in a bacterial cell (or cells) by opposing viral DNA.
Restriction enzyme cuts double or single stranded DNA at a specific recognition nucleotide sequences known as restriction site.
it can't be heated to inactivation,instead you can just try protease K treatment
There main purpose is to cut up viral DNA that invades bacteria. Since they do this at definite sites, depending on which restriction enzyme is used, they can be used in genet…ic recombination exercises. Inserting sections of DNA into bacterial plasmids so that desired products will be made by these bacteria.
restriction enzymes cut the DNA at specific sites called restriction sites. These restriction sites are 6 - 8 base pairs long and occur at different frequencies on the DNA