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Crystal violet is a hexamethyl also known as methyl violet 10B This is much darker than 2B, and often darker than 6B. It is used in biological stainang particularly gram staining together with safranin and iodine. Crystal violet was also used to treat strept throat. Doctors used to swab it on the back of your throat.
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∙ 15y ago
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∙ 9y agoCrystal violet is a staining reagent. The crystal violet stain forms a purple complex within the cell wall. In Gram's Method, the stain is used to differentiate bacteria into groups.
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∙ 11y agoIt is a sugar and it is a nutrient for bacteria that are lactose fermentors
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Crystal violet in the MacConkey agar?
MacConkey's agar refers to a culture medium that is designed to selectively grow Gram-negative bacteria. It contains crystal violet dye, which inhibits certain Gram-positive bacteria.
When white light passes through a prism which will be bent more the red or green light?
In case of visible region, VIBGYOR, violet has the maximum bending.
What is the difference between gram's stain and wright's stain?
Wright's Stain is a mixture of methylene blue and eosin in methanol. Gram's stain is crystal violet, iodine washed with acetone and proofed with a safranin dye to look for gram negative organism.
What is a druce crystal?
crystal
Enumerate the advantages derived from staining bacteria?
Staining bacteria is done by using a dye called crystal violet. This method helps in distinguishing different types of bacteria, as well as see the internal parts.
What is the secondary stain in gram stain procedure?
It is crystal violet & stains all cells purple.
What is gentian violet?
Traditional antifungal drugs such as.gentian violet (also known as crystal violet)
What function of iodine in gram stain?
fixing the stain so that the first dye which is the crystal violet will not be washed away during rinse process.
What function of lugol's iodine in gram staining?
fixing the stain so that the first dye which is the crystal violet will not be washed away during rinse process.
What is crystal violet iodine complex?
In Gram staining procedure during bacterial staining , iodine forms a complex with crystal violet stain which stains Gram positive bacteria blue to violet .
What is the extinction coefficient of crystal violet?
balls
Function of Gram's iodine?
Iodine is used as a mordant in the gram staining procedure to make large crystals when it is used with crystal violet dye. In gram positive cell walls those crystals get stuck and wont get washed off with the alcohol. In gram negative cell walls the crystals are washed out.
Why is Iodine used in Gram staining?
Iodine is added as a mordant to enhance crystal violet staining by forming a crystal violet-iodine complex.
Crystal violet acid-base indicator?
Yes it is.
Function of crystal violet in gram stain?
Well if you stained with only crystal violet, then they would all be violet! If you do a Gram Stain the right way, you end up getting Gram positive being violet or purple and Gram Negative being red or pink.
Function of iodine in Gram stain?
iodine act as a mordant..on the gram positive bacteria which got really thick and abundence of peptidoglycan layer, the crystal violet will fix to the peptidoglycan layer..meanwhile in gram negative bacteria which is lack of peptidoglycan layer, the alcohol or acetone will wash it away
Function of acetone in gram staining?
Acetone is used for decolouring method, washing away the iodine-crystal violet complex formed in gram negative bacteria. so that the gram negative bacteria can be then stained with safranin or fuchsin.
