If your talking about Acid Fast staining (aka Ziehl-Neelsen staining), after the addition of the primary stain (carbol fuchsin) heat is applied in order to facilitate proper staining. Bacteria such as Mycobacterium contain large amounts of lipid substances within their cell wall called mycolic acids. These acids resist staining by ordinary methods such as gram staining (where heat is not applied after primary staining). On application of heat, the stain carbol fuschin penetrates the cell wall and stains the cells pink. Following the secondary staining (methylene blue) the acid fast positive cells appear pink while others are stained blue.
Endospore staining is yet another staining technique where heat is applied after primary staining (malachite green). In this case the spores are impermeable to stains, hence heating favours proper permeation of stain. Endospores appear green while vegetative cells appear red (secondary stain saffranine).
Not all staining procedures involve applying heat after primary staining. However, heat is applied before even beginning the staining procedure. This is called heat fixing, where the cells are fixed so that they are not washed away during the subsequent washing steps.
There are many different purposes of staining depending on the situation. Medical staining helps to show DNA and it's components.
To colorize & enhance trasparent bacteria, which show cell shape & arrangement.
colors the cell making it easier to see.
to make the wood look good and shiny.so the furniture stands out
The primary stain is the stain that will actually stain what you want to see. In the case of the Gram stain, that is the particular structure of the cell wall of the Gram positive bacteria.
the primary stain simply stains the bacterial cell
what is the function of a primary stain
it stains the cells purple
It is essential that primary stain and the counterstain be of contrasting colors so that the target of the primary stain can easily be differentiated on a contrasting background.
Both processes use 2 stains. The Gram staining process uses crystal violet as the primary stain and safranin as the secondary stain. Acid-fast staining uses carbol fuchsin as the primary and methylene blue as the secondary.
Its the primary stain of the procedure. IT stains the Gram positive organisms
Assume that during the performance of this exercise you made several errors in your spore-staining procedure. In each of the following cases indicate how your microscopic observations would differ from those observed when the slides were prepared correctly . A. you used acid-alcohol as the decolorizing agent . B. you used safranin as the primary stain and malachite green as the counterstain C. you did not apply heat during the application of the primary stain · A. Normally tap water is used as the decolorizing agent to wash off excess stain. When you use acid-alcohol, it decolorizes the cells and the stain is removed. · B.When you use safranin as the primary stain and malachite green as the secondary stain, the cells will stain green and the spores will stain red. · C.When heat is not applied during the application of the primary stain, the spores are not stained and they appear colorless.
if you didnt apply heat or Clorox, then the stain would not go away completely and we all don't want that!
It is essential that primary stain and the counterstain be of contrasting colors so that the target of the primary stain can easily be differentiated on a contrasting background.
Heat is the mordant used in the spore stain, it fixes the primary stain.
Iodine work as a modrant which helps in fixing the stain properly by making a compled compoun of stain and cell wall.
Both processes use 2 stains. The Gram staining process uses crystal violet as the primary stain and safranin as the secondary stain. Acid-fast staining uses carbol fuchsin as the primary and methylene blue as the secondary.
Its the primary stain of the procedure. IT stains the Gram positive organisms
Its the primary stain of the procedure. IT stains the Gram positive organisms
It is crystal violet & stains all cells purple.
Simply you can use some stain removal that available in markets. Or try hot water. Hot water is primary good stain removal process.
Assume that during the performance of this exercise you made several errors in your spore-staining procedure. In each of the following cases indicate how your microscopic observations would differ from those observed when the slides were prepared correctly . A. you used acid-alcohol as the decolorizing agent . B. you used safranin as the primary stain and malachite green as the counterstain C. you did not apply heat during the application of the primary stain · A. Normally tap water is used as the decolorizing agent to wash off excess stain. When you use acid-alcohol, it decolorizes the cells and the stain is removed. · B.When you use safranin as the primary stain and malachite green as the secondary stain, the cells will stain green and the spores will stain red. · C.When heat is not applied during the application of the primary stain, the spores are not stained and they appear colorless.
if you didnt apply heat or Clorox, then the stain would not go away completely and we all don't want that!
counterstains are selected to be contrasting color so that the target of the primary stain can easily be differentiated on a contrasting background. This makes life easier, when, for example you need to count the number of nuclei in a smear, or number of gram positive bacteria in a mixed population.
The counter-stain allows you to see all the structures that were not stained with the primary stain. Without the counter-stain, all you would see is the purple-stained structures (nucleus, some cytoplasmic proteins), but you would have a difficult time observing the cell membrane and many cytoplasmic structures.