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Steps in making a smear

Updated: 12/18/2022
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12y ago

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Smear is one of the forms in which organisms are viwed under the light microscope in microbiology, the other form is as wet mounts, remember that smear is used to view killed organisms the steps in making smear are as follow: 1-Get a grease free slide and a wire loop. 2-sterilize the wire loop by flaming. 3-deep the wire loop in a normal saline. 4-touch the slide with the wire loop, there will be a drop of normal saline on the slide. 4-sterilize the wire loop again and touch a small part of a colony in petri dish, then mix it with the normal saline on the slide. 5-allow it to air dry. 6-pass it through an open flame three times(heat fixation). Then view under the microscopes. The heat fixation is done in order to: 1-to fix the smear to the slide. 2-to kill the organism 3-to alter the organism so that it can accept stain readily.

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