0
Supernatant is the clear fluid above a precipitate or sediment. Pellet is a small rounded object, ball, or spherical body.
Wiki User
∙ 9y ago
Add your answer:
Earn +20 pts
The order in which cellular components will be found in the pellet when homogenized cells are treated with increasingly rapid spins in a centrifuge?
The correct order in which cellular component will be found in the pellet when homogenized cells are treated with increasingly rapid spins in a centrifuge is a nucleus, mitochondria, and ribosomes. Bound ribosomes generally synthesize membrane proteins and secretory proteins.
What is the space between fuel pellet and fuel rod cladding?
gap
How can the value of a j c Higgins 22 cal pellet pisol 12619380 be found?
Check "Blue Book of AirGuns 7th edition" Both Daisy and Crosman made Airguns under the J C Higgins name for Sears.
Why do pellets build up in the fire box of my pellet stove?
Pellets will build up for numerous reasons. First thing I would check is the air flow settings on your pellet stove. If it is too low not providing enough air the pellets will not burn fast enough causing them to pile up. Another possibility is that the feed rate is set too high on your stove. I would recommend cleaning out your stove really well, removing all the ash and trying to see if they pile up again. You really have to mess with your feed rate and your air controls to get the proper settings where it runs smooth.
What is pellet stove auger gear motor?
The auger is like a big screw. As the screw turns pellets travel from supply bin to fire along the shaft. The auger turns because it is connected to a motor that is geared down from its rotational speed to a slower speed so pellets are delivered in a timely way to the fire and not shot like bullets out of the stove.
Why did DNA in Hershey-Chase experiments end up in the supernatant?
It didn't. The DNA ended up in the pellet-like bacteria that was at the bottom of the centrifuge. The protein capsids ended up in the supernatant because they weren't injected into the bacteria cells. The supernatant was separated from the bacteria cells because the bacteria was heavier and fell to the bottom.
In the fractionation of homogenised cells using centrifugation the primary factor that determines whether a specific cellular component ends up in the supernatant or the pellet is?
the size and weight of the component
What does the term supernatant refer to scientifically?
The term supernatant scientifically refers to lying above a precipitate or sediment. Supernatant scientifically also refers to floating on the surface of a liquid.
What is the color of supernatant liquid in decantation?
The color is different for each system; if the approx. pure water is the supernatant, this is colorless.
What is the solution that remains after a precipitate has formed?
Supernatant.
The order in which cellular components will be found in the pellet when homogenized cells are treated with increasingly rapid spins in a centrifuge?
The correct order in which cellular component will be found in the pellet when homogenized cells are treated with increasingly rapid spins in a centrifuge is a nucleus, mitochondria, and ribosomes. Bound ribosomes generally synthesize membrane proteins and secretory proteins.
What is the use of resuspension of supernatant in fresh medium?
LB medium
Why is it necessary to test for complete precipitation if a supernatant solution is going to be subsequently tested for ions?
The presence of unprecipitated ions in the supernatant may react with subsequent reagents added and obscure the results of tests.
When centrifuging a mixture the lighter material in the solution on top is called?
the supernatant
What is the difference between supernatant and filtrate?
supernatant - liquid above and solid in the bottom, the supernatant is the liquid above that is going to be poured off (to separate the solid and the liquid) filtrate - it is also the liquid part but the solid and liquid is separated differently, for example coffee the filter paper holds on to residue (solid) and the liquid part (filtrate) passes I think that is the difference.
What is the function of ethanol in RNA extraction?
This wash step allows you to centrifuge the sample and collect a "clean" RNA pellet, after discarding the supernatant that contained contaminating salts and proteins. When isolating and purifying RNA, 75% ethanol is used as a wash solution because RNA is a precipitate (solid) in this percentage of ethanol, while most proteins and salts remain in solution (are soluble). At a lower % ethanol, both the RNA and the proteins would be soluble, so you would not be able to separate them. At a higher % ethanol, both the RNA and salts would remain in the pellet, so you would not be able to separate the salts from your RNA. Prior to the wash step, you probably added 100% ethanol to your sample, so the final total concentration of ethanol was 75%. This step is where the RNA precipitates out of solution. You would then centrifuge the sample and discard the supernatant, as above. In the wash step, you are merely using the same solution (75% ethanol) to wash the RNA pellet you created in the previous step.
What is a centrifuge?
A centrifuge generally uses centrifugal force to separate the desired product, which can either be proteins or nucleic acids, from the suspension made of insoluble fine solids in liquids. The desired product can be either in the supernatant (the clear liquid obtained as a result of centrifugation) or the pellet (residue from centrifugation sedimented at the bottom). A centrifuge uses the idea of centrifugal force by rapidly spinning a sample around many times. This causes the sample, upon completion, to be separated into its highest density components at the bottom of the centrifuge to its lowest density components at the top. This makes doing certain things easier, like decanting supernatant (liquid) or collecting specific layers from a sample.
