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Thin-layer chromatography consists of a stationary phase immobilised on a glass or plastic plate and a solvent as mobile phase. The sample, either liquid or dissolved in a volatile solvent, is deposited as a spot on the stationary phase. The constituents of a sample can be identified by simultaneously running standards with the unknown spot. One edge of the plate is then vertically placed in a solvent reservoir and the solvent moves up the plate by capillary action. When the solvent front reaches the other edge of the stationary phase, the plate is removed from the solvent reservoir. The separated spots are then visualized with ultraviolet light or by a suitable reaction procedure....:)
Wiki User
∙ 11y ago
Wiki User
∙ 14y agoThin Layer Chromatography (TLC) is a method of separating the components that are in a sample of a mixture.
Wiki User
∙ 14y agoElectrophoretic separation of a mixture of amino acids and peptides on a thin layer of silica gel.
Wiki User
∙ 11y agothey are layers and they act like layers
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What is the mechanism of separation in thin layer chromatography?
i think mechanism is that,,thin layer chromatography is basically separation of mixture,and sample might adsorb on silics surface.here is adsorption mechanism
How is paper chromatography used in everyday life?
it can be used in everyday life by liquid chromatography, gas chromatography, thin-layer chromatographyand paper chromatography.
Is Thin Layer Chromatography a good way to analyzes carbohydrates?
yer no
What is the name of the method used to separate dyes?
Chromatography is the method used to separate a mixture of dyes or inks.
What group of compound can be identified using iodine in thin layer chromatography?
hydrocarbon
What has the author Bernard Fried written?
Bernard Fried has written: 'Thin-layer chromatography' -- subject(s): Thin layer chromatography
Are paper and thin layer chromatography types of planer chromatography?
yes.
What are 3 types of chromatography?
1. thin -layer chromatography 2. gas chromatography 3. liquid chromatography
What is the mechanism of separation in thin layer chromatography?
i think mechanism is that,,thin layer chromatography is basically separation of mixture,and sample might adsorb on silics surface.here is adsorption mechanism
How is paper chromatography used in everyday life?
it can be used in everyday life by liquid chromatography, gas chromatography, thin-layer chromatographyand paper chromatography.
Is Thin Layer Chromatography a good way to analyzes carbohydrates?
yer no
What has the author F Geiss written?
F. Geiss has written: 'Fundamentals of thin layer chromatography (planar chromatography)'
What is the name of the method used to separate dyes?
Chromatography is the method used to separate a mixture of dyes or inks.
What are the Advantages of column chromatography over thin layer chromatography?
One is faster and more flexible, the other is a bit heavier
What group of compound can be identified using iodine in thin layer chromatography?
hydrocarbon
Distinguish between TLC and hptlc?
TLC is Thin layer Chromatography while HPTLC is High Performance Thin Layer Chromatography HPTLC is Automotive ,It has Scanner,Injector,Developer -al automated and controleed generally by Software
Which chromatographic process has minimal equipment and space requirements?
(TLC) Thin-Layer Chromatography
