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What are the different types of polymerase chain reaction techniques?
Wiki User
∙ 11y ago
types of pcr: AFLP -PCR.
Allele-specific PCR.
Alu-PCR.
Assembly -PCR.
Assemetric -PCR.
Colony -PCR.
Helicase dependent amplification.
Hot start pCR.
Inverse -PCR.
Insitu -pCR.
ISSR-PCR.
RT-PCR(REVERSE TARNSCRIPTASE).
REAL TIME -PCR
Wiki User
∙ 14y ago
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A small amount of DNA obtained from a mummy or frozen remains of a human may be cloned in order to clone small amounts of DNA what needs to be used to generate larger qualities of DNA?
To ramp up the DNA into a large enough amount to work with you would use polymerase chain reaction technique. PCR. By using the enzymes found in certain extremeophiles one alternately heats and cools the DNA solution with this extremeophile polymerase included and it ramps up the amount of DNA so you have a useful bit of DNA for insertion into a cloning vector.
What is the working principal behind RT PCR?
Reverse transcription polymerase chain reaction (RT-PCR), is a variant of polymerase chain reaction (PCR) commonly used in molecular biology to detect RNA expression. RT-PCR is used to qualitatively detect gene expression through creation of complementary DNA (cDNA) transcripts from RNA.Even though both techniques, RT-PCR and PCR, produce multiple copies of a particular DNA through amplification, the applications of the two techniques are fundamentally different. The most common PCR technique is used to exponentially amplify target DNA sequences. Meanwhile, RT-PCR is used to clone expressed genes by reverse transcribing the RNA of interest into its DNA complement through the use of reverse transcriptase enzymes. Subsequently, the newly synthesized cDNA by RT-PCR is amplified using traditional PCR technique.Usually, RT-PCR is often confused with real-time polymerase chain reaction (qPCR) by students and researchers alike, but they are quite separate and distinct techniques.
How could you use genetic engineering techniques to make transformed bacteria that produce the enzyme?
Extract DNA from the cells of people who can make the digestion enzyme. Cut the DNA with restriction enzymes to cut out the gene that codes for the enzyme. Use gel electrophoresis to locate the gene. Then, use polymerase chain reaction to make copies of the gene. Choose a plasmid that has an antibiotic-resistance genetic marker, and cut the plasmid with the smae restriction enzyme use to cut out the hyman gene. Insert the copies of the human gene into the plasmids. Allow bacterial cells to take in the plasmids. Select for transformed bacteria by growing them in a culture containing the antibiotic. These bacteria will make the digestion enzyme.
How can you test a food to find out if it contains material derived from a genetically modified organism?
This can be done many ways. One is using an antibody-based test such as the enzyme-linked immunosorbent assay (ELISA), which can detect the proteins that are produced by GM crops. However, the ELISA is not useful for testing foods that is highly processed, because the proteins most likely destroyed & different GM foods produce different proteins. Another way is to use polymerase chain reaction (PCR) to look at the DNA sequence common in GM foods. DNA is more resistant than proteins in processing & can be extracted from heavy processed foods. It is the GMO DNA sequences that's being looked for & tested.
What is Nuclear reactors and it's parts and functions?
it is a device in which chain reaction is initiate or controlled $generate heat energy typcially for power
Which process uses bacteria to copy DNA?
Polymerase chain reaction
What is the source of the polymerase used in polymerase chain reaction?
Taq polymerase, the enzyme used frequently in Polymerase Chain Reaction, is extracted from Thermophilus aquaticus, a thermophilic bacteria.
Process used to amplify small DNA samples?
I is known as Polymerase Chain Reaction (PCR)
What does PCR stand for?
Polymerase Chain Reaction
What is PCR short for?
A polymerase chain reaction
What is a method of making many copies of DNA?
Polymerase chain reaction
What ingredients are needed to start polymerase chain reaction?
7yutii
What the second step in the Polymerase chain reaction process?
anealing
Which is a method of making copied of DNA?
polymerase chain reaction
PCR stands for?
PCR stands for Polymerase Chain Reaction.
What is the process in which you bring about a polymerase chain reaction?
To bring about a polymerase chain reaction DNA sequences are placed in .2-.5ml reaction tubes and then placed in a thermal cycler. To achieve the reaction the sequences must undergo 20-40 temperature changes.
What is used to make many copes of DNA?
Polymerase chain reaction
