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the column is some strange scientist
Gel filtration or gel permeation is a process by which molecules can be separated according to their size(molecular weight) and sometimes shape. small molecules get trapped and slowed down in the pores of the beads but large molecules simply flow down the column..therefore larger molecules come out first.
The purification in molecular sieve chromatography is dependent on the size of the molecules. The small molecules will enter into pores of gel while large molecules will be excluded from the pores.
The hemoglobin can pass through the gel first because it has a higher molecular weight, or larger molecule which could not pass through the pores of the beads in the gel, while the riboflavin would flow slower.
Filtration separates particles based on size.
the column is some strange scientist
Tibor Kremmer has written: 'Gel chromatography' -- subject(s): Gel permeation chromatography
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in gel filtration the volume of eluent is what moves compounds in and out of the pores in the matrix.A large volume of sample means the sample components will not be introduced to the matrix as a tight "plug" and the sample will slowly spread into the matrix meaning the beginning and the ending of the sample will be far apart meaning the sample will be spread out over the gel matrix resulting in poor resolution of that component from any other.
Gel filtration or gel permeation is a process by which molecules can be separated according to their size(molecular weight) and sometimes shape. small molecules get trapped and slowed down in the pores of the beads but large molecules simply flow down the column..therefore larger molecules come out first.
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the column is some strange scientist
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Liquid chromatography separation of multicomponant system according to diffrent interaction of dissolved componant with stationary phase. the stationary phase and mobile phase is very wide range
The purification in molecular sieve chromatography is dependent on the size of the molecules. The small molecules will enter into pores of gel while large molecules will be excluded from the pores.
The hemoglobin can pass through the gel first because it has a higher molecular weight, or larger molecule which could not pass through the pores of the beads in the gel, while the riboflavin would flow slower.