Bacterial staining: These are the methods for staining various parts of bacterial cell.
1. Gram staining: Used for primary identification of two major groups of bacteria - Gram Positive and Gram Negative.
2. Cell wall staining: shows cell wall of bacteria.
3. Nuclear staining: Though bacteria do not possess true Nucleus, an area high concentration of Nucleic acids can be stained.
4. Lipid stain
5. Spore stain
6. Flagella
7. Capsule
8.Meta chrimatic granules.
9.. Spirocheate staining: this method is used to show presence of spirocheates in a smear.
stains are salt of a +ve and -ve ion one of which is coloured and is known as chromophore. stains are frequently used in Biology and medicine to highlight structures in biological tissues for viewing often with the aid of microscope. Stains may be used to define and exammine bulk tissues, cells populations or organelles within individual cells. stains are generally classified on the basis of ion to which they intract as acidic, basic and neutaral. e.g. methylene blue, eosin, basic fuschin, malachite green etc.
Stains are salt composed of a (+)ve and a (-)ve ion one of which is coloured and is known as chromophore.
In which dye colour is in the (+)ve ion is called Basic dye & in Acidic dye colour is in the (-)ve ion.
Basic dyes:
Methyleneblue, crystal violet, Safranin, Malachitegree etc. are the basic dye which bind to negatively charged organ such as nucleic acid &protein .
Acidic dyes:
Eosin , acid fuschinetc. etc.are the acidicdye which bind to
Positively charged cell structures.
Common biological stains
• Crystal violet:
when combined with a suitable mordant, stains cell walls purple. Crystal violet is an important component in Gram staining
• Eosin
used as a counterstain. imparting a pink or red colour to cytoplasmic material, cell membranes, and some extracellular structures. It also imparts a strong red colour to red blood cells.
• Ethidium bromide:
intercalates and stains DNA, providing a fluorescent red-orange stain
• Fuchsin
used to stain collagen, smooth muscle, or mitochondria Iodine
used as an indicator for starch. When starch is mixed with iodine in solution, an intensely dark blue color develops, representing a starch/iodine complex.
The three basic stains are:
1. crystal violet
2. carbolfuchsin
3. methylene blue
These are basic stains because they have the ability to bind with the bacteria using ionic interactions. This means that the stains have postively charged groups that bind with the bacteria's negatively charged groups.
Stains are dirty marks that are not easily removed.
crystal violet, methylene blue, and safranin
Crystal violet
Methylene blue
Safranin
We just did this today in honors bio 2 hope it helps.
Methylene Blue, Crystal Violet, and carbon fuhsion
Too
The three basic parts of an atom is the nucleus,protons and neutrons
which of the following is not one of the three basic types of major joints in the body
There are several biological stains that can be used for plants. Some of the usual stains used on plants are toluidine blue, iodine, and Eosin Y.
Mass, volume, and density
methylene blue crystal violet carbol fuchsin
Regarding laundry: Blood, grass, oil (car and cooking) and paint.Otherwise, stains are used to change colors in biochemical uses, wood work, art and metal work.
Acidic,basic and neutral stains
the foundation
willly
they are called primary colours.
in philippines, they used chlorine in removing stains
Introduction to basic techniques in microscopy involves light microscopy, laser scanning, types of dyes, the cell, electron microscopy, differential interface microscopy, histological stains and histochemical stains.
Planning, control, apt, databases, stains, porter, ganado
A basophil is a cell which has granules stained by basic stains, especially granular leukocytes.
name three basic dimensions of orthographic drawing
Planning, control, apt, databases, stains, porter, ganado