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What is the function of the mobile phase for developing a chromatogram?
Wiki User
∙ 12y ago
stationary phase is the solid called the filter paper and mobile phase is the liquid or organic solvent present in the developing tank or beaker by ayesha zulfiqar
Wiki User
∙ 11y ago
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What is the purpose of the filter paper inside the developing chamber in chromatography?
Paper is the (solid) stationary phase, 'sucking' up the liquid, mobile (= transporting) phase
What is stationary phase and mobile phase?
mobile phase is the phase that consist of the analyte and stationary phase is the phase that is standstill
Why the retention time will decrease if the polarity of mobile phase increase in normal phase HPLC?
because in normal phase HPLC mobile phase is non polar and stationary phase is polar. Most of the compound of interest are polar, if you increase the polarity of mobile phase compound of analyte will stay in mobile phase and will elute faster and retention time will be shorter.
What is chromatography and its uses?
finding concentrations gas chromatogram of gasolineion chromatogram of Orange Juiceeach peak corresponds to a separate component in the mixturearea of each peak is proportional to concentrationchemical fingerprinting species identification "killer" bees can be distinguished from native bees by comparing gas chromatograms of cuticle extractstracing contraband sourcesdetecting drugs in urine
What is the mobile phase of ion exchange chromatography?
The mobile phase as indicated is the moving phase. Either the mobile or stationary phase is polar and the other is Non-polar. A common polar phase is Methanol, and non-polar is hexane
What would happen if the beaker is left uncovered during the development of the chromatogram?
Bad things happen. The mobile phase evaporates and thus it's composition changes. The vapor phase above the chromatogram is not saturated or at equilibrium, and this affects the movement of the sample on the solid phase.
What is the systm volume and what is the dead volume and what is the wide volume and what is the delay volme in HPLC?
system volume is amount of mobile phase travel from reservior to tip of the colomn . dead volume is the amount of mobile phase present in colomn. delay volume is amount of mobile phase used in at the time of injection and the time of detection of chromatogram.
What are mobile phase and stationary phase?
stationary phase is the solid called the filter paper and mobile phase is the liquid or organic solvent present in the developing tank or beaker by ayesha zulfiqar
What is the purpose of the filter paper inside the developing chamber in chromatography?
Paper is the (solid) stationary phase, 'sucking' up the liquid, mobile (= transporting) phase
What is stationary phase and mobile phase?
mobile phase is the phase that consist of the analyte and stationary phase is the phase that is standstill
What is mobile phase?
The mobile phase as indicated is the moving phase. Either the mobile or stationary phase is polar and the other is Non-polar. A common polar phase is Methanol, and non-polar is hexane
Why is it important to cover the developing chamber in paper chromatography?
For thin layer or paper chromatography, this can be a very important step, especially if one or more of the components in the mobile phase have very high vapor pressures (or low boiling points). Without the equilibration, as the mobile phase starts to move up the plate, the more volatile components will evaporate into the air space above the mobile phase faster than the less volatile ones. This is change the composition of the mobile phase (not much, but sometimes enough to change the Rf's of the unknown spots and standards). If the mobile phase consists of only water or perhaps high boiling solvents, the equilibration step is not as important. If the mobile phase contains Methylene chloride, Diethyl ether or similar solvents, then the equilibration step can be very important in terms of reproducibility.
Why the retention time will decrease if the polarity of mobile phase increase in normal phase HPLC?
because in normal phase HPLC mobile phase is non polar and stationary phase is polar. Most of the compound of interest are polar, if you increase the polarity of mobile phase compound of analyte will stay in mobile phase and will elute faster and retention time will be shorter.
Mobile phase in relation to chromatography?
a moving or mobile phase is a mixture you want to separate , dissolved in a solvent.
What is chromatography and its uses?
finding concentrations gas chromatogram of gasolineion chromatogram of Orange Juiceeach peak corresponds to a separate component in the mixturearea of each peak is proportional to concentrationchemical fingerprinting species identification "killer" bees can be distinguished from native bees by comparing gas chromatograms of cuticle extractstracing contraband sourcesdetecting drugs in urine
What is the mobile phase of ion exchange chromatography?
The mobile phase as indicated is the moving phase. Either the mobile or stationary phase is polar and the other is Non-polar. A common polar phase is Methanol, and non-polar is hexane
What is the difference between reverse phase and normal phase chromatography?
Normal Phase: It has a polar stationary phase and a non-polar mobile phase.Reverse Phase: It has a non-polar stationary phase and a moderately polar mobile phase
