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What are the first and last letters of the Greek alphabet?

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The first and last letters of the Greek alphabet are Alpha and Sho

There are 32 letters:
alpha, beta, gamma, delta, epsilon, digamma, stigma, zeta, heta, eta, theta, iota, yot, kappa, lamda, mu, nu, xi, omicron, pi, san, koppa, rho, sigma, tau, upsilon, phi, chi, psi, omega, sampi, sho
Source:
list of all symbols tagged *GREEK*LETTER* in allkeys.txt on Unicode website
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Is beta the last letter in the Greek alphabet?

No. Beta is the second letter of the Greek alphabet. The 24th (last) is omega (Ω).

What are the first and last nations alphabetically?

Within the criteria of Internationally recognised Sovereign states, and if we use the customary English spelling format, and omit the legal style, such as 'Republic of…' 

Why are there 24 letters in the Greek alphabet?

It's a hard question to answer. Because Greeks today dropped:   digamma, stigma, heta, yot, san, koppa, sampi, sho    All alphabets are designed to include the mini