Restriction enzymes can act only on double strand DNA . Restriction enzyme recognizes and hydrolyzes the backbone of DNA between deoxyribose and phosphate groups at or near the restriction sites. This leaves a phosphate group on the 5` ends and a hydroxyl on the 3` end of both the strands . Thus digestion with restriction enzymes results in the fragmentation of the double stranded DNA molecule.
TaqI's restriction site is:TCGAAGCT
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You use the same enzyme inn order to get the same restriction and binding sites.
Effect
A highly specific 'active site'.
Such an enzyme is called a restriction endonuclease
TaqI's restriction site is:TCGAAGCT
Restriction enzyme cuts DNA strand at specific locations Restriction enzyme cuts DNA strand at specific locations
Yes?
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Topoisomerase is not a restriction enzyme but an enzyme that keeps unwound DNA from tangling while it is being replicated.
Restriction enzymes are produced by bacteria to help destroy foreign, invading DNA, such as the DNA of bacteriophage (a virus that infects bacterial cells). Every restriction enzyme comes with a methylase enzyme, or more specifically, a DNA methyltransferase. The methylase enzyme methylates (adds a methyl group) to the restriction endonuclease site on the cell's own DNA, which protects the sites from the restriction enzyme so that it does not degrade its own DNA.
You use the same enzyme inn order to get the same restriction and binding sites.
Restriction enzymes.
a Restriction Enzyme
Effect
restriction endonuclase enzyme (made in bacterial plasmids)