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What are the principles of restriction enzyme use in removing sections of the genome?
Wiki User
∙ 12y ago
Restriction enzymes can act only on double strand DNA . Restriction enzyme recognizes and hydrolyzes the backbone of DNA between deoxyribose and phosphate groups at or near the restriction sites. This leaves a phosphate group on the 5` ends and a hydroxyl on the 3` end of both the strands . Thus digestion with restriction enzymes results in the fragmentation of the double stranded DNA molecule.
Wiki User
∙ 12y ago
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What is the restriction site of the restriction enzyme TaqI?
TaqI's restriction site is:TCGAAGCT
How do you identify restriction enzyme?
By playing with you.
Why did you cut both segments of DNA with the same restriction enzyme?
You use the same enzyme inn order to get the same restriction and binding sites.
How does enzyme restriction limit the affect of a virus?
Effect
What is opened by a restriction enzyme?
A highly specific 'active site'.
An enzyme that cuts double-stranded DNA at specific nucleotide sequences?
Such an enzyme is called a restriction endonuclease
What is the restriction site of the restriction enzyme TaqI?
TaqI's restriction site is:TCGAAGCT
Hi does any one know if I want to put some restriction enzyme close to cDNA should I put some nucleotide befor this restriction enzyme?
Restriction enzyme cuts DNA strand at specific locations Restriction enzyme cuts DNA strand at specific locations
Restriction enzyme digestion?
Yes?
How do you identify restriction enzyme?
By playing with you.
Is topoisomerases belong to restriction enzymes?
Topoisomerase is not a restriction enzyme but an enzyme that keeps unwound DNA from tangling while it is being replicated.
Why restriction enzyme cannot cut its own DNA?
Restriction enzymes are produced by bacteria to help destroy foreign, invading DNA, such as the DNA of bacteriophage (a virus that infects bacterial cells). Every restriction enzyme comes with a methylase enzyme, or more specifically, a DNA methyltransferase. The methylase enzyme methylates (adds a methyl group) to the restriction endonuclease site on the cell's own DNA, which protects the sites from the restriction enzyme so that it does not degrade its own DNA.
Why did you cut both segments of DNA with the same restriction enzyme?
You use the same enzyme inn order to get the same restriction and binding sites.
Which enzyme is used to produce RFLPs?
Restriction enzymes.
What tool to use when cutting plasmid?
a Restriction Enzyme
How does enzyme restriction limit the affect of a virus?
Effect
Which enzyme is Known as Genetical or Genetic Knife?
restriction endonuclase enzyme (made in bacterial plasmids)
