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If the following steps are used to produce monoclonal antibodies What is the fourth step?
Brdavis
1. A mouse is injected with a specific antigen that will induce antibodies against that antigen.
2. The spleen of the mouse is removed and homogenized into a cell suspension. The suspension includes B cells that produce antibodies against the injected antigen.
3. The spleen cells are mixed with myeloma cells that are capable of continuous growth in culture but have lost the ability to produce antibodies. ( Some of the antibody producing spleen cells and myeloma cells fuse to form hybrid cells. These hybrid cells are now capable of growing continuously in culture while producing antibodies.
4. The mixture of cells is placed in a selective medium that allows only hybrid cells to grow.
5. Hybrid cells proliferate into clones called hybridomas. The hybridomas are screened for production of the desired antibody.
6. The selected hybridomas are then cultured to produce large quantities of monoclonal antibodies.
Wiki User
∙ 12y ago
Wiki User
∙ 14y agoculture the hybridoma?
pg.508 in Microbilogy an introduction 10th ed
tortora ,funke, case
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