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Q: What might be some limitations to paper Chromatography?
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What are some possible alternatives for paper chromatography?

Coffee filter paper can be used. It does not produce results as good as chromatography paper, but it still works.


What is paper chromatography?

Paper chromatography is a technique used to separate a mixture into its component molecules. The molecules migrate, or move up the paper, at different rates because of differences in solubility, molecular mass, and hydrogen bonding with the paper.


Uses for paper chromatography?

They are used in many scientific studies to identify unknown organic and inorganic compounds. They are also used in crime scene investigation, DNA and RNA sequencing, among others. Essentially, any solution can be separated through some form of chromatography.


What is meant by tailing a spot in Chromatography?

Chromatography is a method of analyzing the contents of a mixed substance. It is performed by dissolving the substance in a suitable solvent. A drop or spot of the resulting liquid is deposited near the edge of a piece of absorbent paper, such as blotting paper or special chromatography paper. The strip of paper with the spot at the bottom is then suspended over a solvent with the bottom edge of the paper in the solvent. As the solvent soaks upwards, the solvent carries some particles upwards and away from the spot. Different compounds within the mixture will travel at different rates and eventually the strip of paper will show different bands of colors, separating out and indicating some of the various components that were in the original substance. 'Trailing a spot' is a short term for this laboratory process.


What is a suitable solvent for paper chromatography?

In paper chromatography n-butanol:acetic acid:water mixture in the ratio 4:1:1 is used as a solvent whereas in case of thin layer chromatography(TLC) petroleum ether and acetone in the ratio 9:1 is used as a solvent. Also, in some cases, DCM(dichloromethane is used as a solvent.

Related questions

What are some possible alternatives for paper chromatography?

Coffee filter paper can be used. It does not produce results as good as chromatography paper, but it still works.


How can substances be separated?

gas-liquid, paper, or column chromatography are some examples


In chromatography why do some colours not spread up the paper?

They do not move up because the aren't soluble the solvent


What is paper chromatography?

Paper chromatography is a technique used to separate a mixture into its component molecules. The molecules migrate, or move up the paper, at different rates because of differences in solubility, molecular mass, and hydrogen bonding with the paper.


Uses for paper chromatography?

They are used in many scientific studies to identify unknown organic and inorganic compounds. They are also used in crime scene investigation, DNA and RNA sequencing, among others. Essentially, any solution can be separated through some form of chromatography.


What is meant by tailing a spot in Chromatography?

Chromatography is a method of analyzing the contents of a mixed substance. It is performed by dissolving the substance in a suitable solvent. A drop or spot of the resulting liquid is deposited near the edge of a piece of absorbent paper, such as blotting paper or special chromatography paper. The strip of paper with the spot at the bottom is then suspended over a solvent with the bottom edge of the paper in the solvent. As the solvent soaks upwards, the solvent carries some particles upwards and away from the spot. Different compounds within the mixture will travel at different rates and eventually the strip of paper will show different bands of colors, separating out and indicating some of the various components that were in the original substance. 'Trailing a spot' is a short term for this laboratory process.


What are the advantages and limitations of column chromatography over thin layer chromatography?

TLC. The mobile phase is a liquid, the stationary phase is a solid. Useful for seperating and comparing mobility of solids and some liquids dissolved in the mobile phase by their affinities to the solid phase relative to the mobile phase. GLC. The mobile phase ia s gas, the stationary phase is a liquid on a solid support. same concept as TLC. useful for seperating gases by their affinities to the stationary phase...the mobility can then be compared to known compounds for possible identification.


How do you separate blue dye and red dye?

Chromatography-This is when you put dye on a piece of special paper and put it in some water. When the water is soak up the dye will separate.


What is a suitable solvent for paper chromatography?

In paper chromatography n-butanol:acetic acid:water mixture in the ratio 4:1:1 is used as a solvent whereas in case of thin layer chromatography(TLC) petroleum ether and acetone in the ratio 9:1 is used as a solvent. Also, in some cases, DCM(dichloromethane is used as a solvent.


How can paper chromatography be used to separate pigments based on their chemical and physical properties?

It is an appropriate technique to use because it separates the pigments, so one can see which pigments are present, even if some pigments are normally hidden to the naked eye.


How can you separate a single color from ink?

a process called chromatography. you put some ink on spetial paper on a line dip it in water just below the line. the ink should then seperate.


Why does different colour dyes move different speeds up charmatograhy paper?

What is charmatograhy paper? Just kidding! I assume you mean chromatography paper. Well, the answer to your question lies in the solubility of the different colour dyes. The more soluble the dye the faster it will travel across the paper. Also, some substances react better with the paper but I'm not sure that is the right answer.