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A restriction enzyme (also known as restriction endonuclease) is protein which cuts DNA up at specific sequences (called restriction sites) in a genome. For example, the commonly used restriction endonuclease EcoRI recognizes every DNA sequence GAATTC and cuts at the point between the guanine and the adenine in that sequence, forming blunt ends (or straight, even ends). Interestingly and coincidentially, the restriction site for most restriction enzymes are genetic palindromes (the sequence reads exactly the same backwards on the complementary strand). In the case of EcoRI, the two complementary DNA strands for the restriction site are:
5'-- GAATTC --3'
3'-- CTTAAG --5'
After this DNA sequence is cut, it might look something like this:
5'-- G AATTC --3'
3'-- C TTAAG --5'
Wiki User
∙ 13y ago
Wiki User
∙ 14y agoThere are many restriction enzymes found in biological system. Few of them are EcoRI, BamHI, HindIII etc.
Wiki User
∙ 8y agoWhen a restriction enzyme cuts DNA into pieces they are called restriction fragments.
Wiki User
∙ 14y agoendonuclease
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What is the restriction site of the restriction enzyme TaqI?
TaqI's restriction site is:TCGAAGCT
How do you identify restriction enzyme?
By playing with you.
How restriction enzyms are use in DNA recommination research?
they used to cut the DNA at the specific site. For example: BamHI is a restriction enzyme that cuts between the given recognition site:
Why did you cut both segments of DNA with the same restriction enzyme?
You use the same enzyme inn order to get the same restriction and binding sites.
Why is it important to use the same restriction enzyme for both cells in recombinant DNA?
Restriction enzymes are endonucleases that digest the DNA at a sequence specific site. Hind III for example cut between two As in the sequence AAGCTT in the both strand forming a sticky end. If you use this enzyme to cut in your vector DNA, you have to use the same enzyme in the insert DNA so as they can ligate by DNA ligation. This is the important use of same restriction enzyme in cloning.
An enzyme that cuts double-stranded DNA at specific nucleotide sequences?
Such an enzyme is called a restriction endonuclease
What is the restriction site of the restriction enzyme TaqI?
TaqI's restriction site is:TCGAAGCT
Hi does any one know if I want to put some restriction enzyme close to cDNA should I put some nucleotide befor this restriction enzyme?
Restriction enzyme cuts DNA strand at specific locations Restriction enzyme cuts DNA strand at specific locations
How do you identify restriction enzyme?
By playing with you.
Restriction enzyme digestion?
Yes?
Is topoisomerases belong to restriction enzymes?
Topoisomerase is not a restriction enzyme but an enzyme that keeps unwound DNA from tangling while it is being replicated.
How restriction enzyms are use in DNA recommination research?
they used to cut the DNA at the specific site. For example: BamHI is a restriction enzyme that cuts between the given recognition site:
Why restriction enzyme cannot cut its own DNA?
Restriction enzymes are produced by bacteria to help destroy foreign, invading DNA, such as the DNA of bacteriophage (a virus that infects bacterial cells). Every restriction enzyme comes with a methylase enzyme, or more specifically, a DNA methyltransferase. The methylase enzyme methylates (adds a methyl group) to the restriction endonuclease site on the cell's own DNA, which protects the sites from the restriction enzyme so that it does not degrade its own DNA.
Why did you cut both segments of DNA with the same restriction enzyme?
You use the same enzyme inn order to get the same restriction and binding sites.
Why is it important to use the same restriction enzyme for both cells in recombinant DNA?
Restriction enzymes are endonucleases that digest the DNA at a sequence specific site. Hind III for example cut between two As in the sequence AAGCTT in the both strand forming a sticky end. If you use this enzyme to cut in your vector DNA, you have to use the same enzyme in the insert DNA so as they can ligate by DNA ligation. This is the important use of same restriction enzyme in cloning.
What determines how DNA will be cut by a restriction enzyme?
Restriction enzymes cut DNA at specific sites called restriction sites. These restriction sites are typically 6 - 8 nucleotides in length and have a defined set of nucleotide bases. For example, the restriction enzyme Eco R1 cuts at the site: AGGTTC. Therefore, if the target DNA contains the above sequence, Eco R1 is able to cut it within the restriction site. Hence, by looking into the target site and which restriction enzymes are being used, on can make an accurate estimate of where the target DNA will be cut
Which enzyme is used to produce RFLPs?
Restriction enzymes.
