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What is MSA agar?

Updated: 10/25/2022
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13y ago

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Mannitol salt agar or MSA is a commonly used growth medium in microbiology. It contains a high concentration (~7.5%-10%) of salt (NaCl), making it selective for Staphylococci (and Micrococcaceae) since this level of NaCl is inhibitory to most other bacteria.

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Q: What is MSA agar?
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Related questions

Can Streptococcus grow on MSA?

MSA stands for mannitol salt agar. Streptococcus can not grow on this type of agar. This is because it has a very high salt content, which allows only certain microorganisms to grow in it.


Can enterobacter aerogenes grow on mannitol salt agar?

the bacteria dont ferment in MSA, there is no growth on the plat


Does E. faecalis test positive for MSA agar test?

yes it will ferment the mannitol and cause yellow color change.


Can Klebsiella pneumoniae grow on Mannitol salt agar?

No, K. pneumoniae will not grow on Mannitol Salt Agar- gram negative organisms cannot grow on MSA, and K. pneumoniae is gram negative.


Did lactobacillus grow on mannitol salt agar?

Yes. M. smegmatis is a gram + bacteria, and MSA plates select for gram + bacteria.


Does bacillus subtilis ferment acid from mannitol salt agar?

In my result i isolate bacillus sp on mannitol salt agar but i expect that this media has deoration or expaired


You observe growth on your MSA agar plate but it didnt turn it yellow as it should with S.aureas What could have gone wrong?

it could have been contaminated.


Which ingredient in mannitol salt agar supplies carbon?

mannitol is a type of sugar, so it supplies the carbon in the MSA medium


Can serratia marcescens grow in mannitol salt agar?

No it doesn't because it doesn't ferment mannitol. P. mirabilis can actually grow on a MAC agar, but it is colorless. I actually had this lab in Micro260 today.An MSA agar has 7% high salt concentration and provides growth for S. aureus bacteria turning it yellow/gold. An MSA agar is selective for osmotolerant bacteria meaning for bacteria that can grow in different salt concentrations, and it's differential because it can ferment mannitol.


Does micrococcus luteus grow on msa plates?

Yes micrococcus luteus, along with micrococcus roseus both grow on MSA. But, they do not fermente on this agar giving a negative test. However, Staphylococcus aureus grows on MSA and fermentes giving a positive test. *Side note* MSA plate is used to test for G+ coccus. The plate contains salt and salt "loving" bacteria will grow and show yellow colony, example of S. aureus.


Which ingredient supplies carbon in the mannitol salt agar?

mannitol is a type of sugar, so it supplies the carbon in the MSA medium


Is CSS the same as MSA?

What is msa