Mannitol salt agar or MSA is a commonly used growth medium in microbiology. It contains a high concentration (~7.5%-10%) of salt (NaCl), making it selective for Staphylococci (and Micrococcaceae) since this level of NaCl is inhibitory to most other bacteria.
yes it will ferment the mannitol and cause yellow color change.
Yes. M. smegmatis is a gram + bacteria, and MSA plates select for gram + bacteria.
In my result i isolate bacillus sp on mannitol salt agar but i expect that this media has deoration or expaired
yesTypically, MSA is used to isolate and differentiate various staphylcocci, some of which are pathogenic, like S. aureus, or non pathogenic like S. epidermidis. Staph are the only kind of bacteria that will grow on MSA. S. aureus is the only staph that ferments manitol. Meaning, MSA can be used to isolate pathogenic from non-pathogenic staph.source: biology major
yes It can, because MSA grows gram positive bacteria.
MSA stands for mannitol salt agar. Streptococcus can not grow on this type of agar. This is because it has a very high salt content, which allows only certain microorganisms to grow in it.
the bacteria dont ferment in MSA, there is no growth on the plat
yes it will ferment the mannitol and cause yellow color change.
No, K. pneumoniae will not grow on Mannitol Salt Agar- gram negative organisms cannot grow on MSA, and K. pneumoniae is gram negative.
Yes. M. smegmatis is a gram + bacteria, and MSA plates select for gram + bacteria.
In my result i isolate bacillus sp on mannitol salt agar but i expect that this media has deoration or expaired
it could have been contaminated.
mannitol is a type of sugar, so it supplies the carbon in the MSA medium
No it doesn't because it doesn't ferment mannitol. P. mirabilis can actually grow on a MAC agar, but it is colorless. I actually had this lab in Micro260 today.An MSA agar has 7% high salt concentration and provides growth for S. aureus bacteria turning it yellow/gold. An MSA agar is selective for osmotolerant bacteria meaning for bacteria that can grow in different salt concentrations, and it's differential because it can ferment mannitol.
Yes micrococcus luteus, along with micrococcus roseus both grow on MSA. But, they do not fermente on this agar giving a negative test. However, Staphylococcus aureus grows on MSA and fermentes giving a positive test. *Side note* MSA plate is used to test for G+ coccus. The plate contains salt and salt "loving" bacteria will grow and show yellow colony, example of S. aureus.
mannitol is a type of sugar, so it supplies the carbon in the MSA medium
What is msa