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An acid-fast pathogen is a bacteria that is harmful to humans. They have cell walls that contain mycolic acid which is a lipid. Common Gram type staining techniques wont work with these cells. A special stain carbolfuchsin is used to penetrate the wall.

After staining you wash with acid alcohol if the stain remains it is acid fast if it washes out it is non-acid fast.

Mycobacterium tuberculosis is a well known acid-fast pathogen

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