answersLogoWhite

0


Best Answer

The separation behind HPLC is based on the polarity of the compounds.

{Polarity refers to : the greater the difference in electron affinity i.e. electronegativity between atoms in a covalent bond, the more polar the bond.partial negative charges are found on the most electronegative atoms, the others are partially positive.The moleacular electrostatic potential energy of a hydrogen ion at a particular location near a molecule. Negative electrostatic potential corresponds to : partial negative charges , Positive electrostatic potential corresponds to : partial positive charges.}

It is used to analyze,identify,purify and quantify componds.

It has a mobile phase,a stationary phase,detector.The mobile phase is continuously pumped at a fixed flowrate through the system and mixed by the pump.The injector is used to introduce a plug of a sample into the mobile phase without having to stop the mobile phase flow,& without introducing air into the system.

The mixture of components is carried in a narrow band to the top of the column.Some compounds in the sample mixture will have greater preference for stationary phase than the mobile phase and will be retained in the column longer.

POINT TO REMRMBER : "Like attracts Like" i.e if the column is non-polar the compound to elute first will be the most polar one.

Those components that are not retained as strongly as are carried by the mobile phase down the column.

The detector is then used to respond to a physico-chemical property of analyte,this response is digitally amplified and sent to a data system where it is recorded as a 'chromatogram'.



User Avatar

Wiki User

14y ago
This answer is:
User Avatar

Add your answer:

Earn +20 pts
Q: What is basic principle of hplc?
Write your answer...
Submit
Still have questions?
magnify glass
imp
Related questions

How does one apply the HPLC princple?

The HPLC principle is used to separate liquid mixtures into individual parts. It's frequently used in several field such as medicine and law. HPLC is specifically used in sports to test for performance enhancing drugs in urine.


What is the basic principle of radar communication system?

Doppler effect is the basic principle for the radar.


Explain basic principle of analog clamp meter?

basic principle of analogue clamp meter


What is the basic principle of belt conveyer in two words?

The basic principle of a belt conveyor is a # looped pully #


Basic principle of uv?

The basic principle is that UV light is high energy light with shorter wavelength.


How do you distinguised np-hplc and rp-hplc?

NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).


What are the principles of administration?

basic administ


Which one is advanced gc or hplc?

HPLC is advanced


Is combinatorics the most principle form of mathematics?

As basic as combinatorics is, I feel that just the basic knowledge of the recognition of what a number actually is, would be more basic of a principle.


What are the basic principle of work management?

Principle of Risk Variation. Principle of Cost of Capital. Principle of Equity Position. Principle of Maturity of Payment.


What is a basic principle or element?

Carbon


What is the basic principle of concurrency?

What is concurrancy?