I recently completed colony morphology on the E.colispecimen.
The results displayed small colonies that were circular in shape, a diameter of approximately 0.5mm, the elevation of the colonies was seen to be slightly raised with the surface incredibly smooth.
The colonies were also seen to have a pink colour pigmentation in Mac conkey agar due to its lactose fermenter after growth.
The gram stain for these bacteria should be negative rod shaped.
Colonial morphology" This gram negative rod is a common contaminant of vegetable matter which forms shiny colonies with entire margins and convex elevation.
Gram stain: negative
Cell shape: bacillus
Arrangement: single
Oxygen requirements: facultative anaerobes
Habitat: intestinal tracts of humans and animals; water; sewage; soil
Pathogenicity: Commonly called "enterics", they can cause urinary tract infections and nocosomial infections. Their presence is not indicative of fecal contamination, so we run a test called the IMViC test to distinguish
Escherichia coli is shaped like a rod; bacteria with this shape are called bacilli. Salmonella is another example of bacilli.
NLF clony on Mac/SSA and Red colony on XLD
The colony morphology of E. coli on nutrient agar looks lactose negative, cellobiose negative, and sucrose negative. In some colonies, a metallic green color is given off by the specimen.
yellow pin point colony
punctiform!
Bacillus subtilis E. coli Pseudomons fluorenscens streptococcus faecilis most bacteria can grow on nutrient agar
* Colonies are circular, 2-3 nm in diameter with a smooth shiny surface when grown on nutrient agar,milk agar or blood agar for 24 h at 37 degrees * Colonies are often pigmented,though a few strains are unpigmented Got the answers from here http://www.geocities.com/SouthBeach/Port/3008/staphyl.html
The intended use of Nutrient Agar with MUG is for detecting an enumerating E. Coli in water. This lab wasn't looking specifically and only for e.coli. It was looking for other types of coliforms as well. this is my best guess
E.coli does not digest the starch on a starch agar plate, therefore it does not produce amylase making it negative.
Luria agar is Luria broth with the addition of 15 grams per liter agar. Agar is a solidifying agent that makes the liquid media solid at room temperature. Hence, luria agar would be a petri dish of agar while luria broth would be liquid to go in a tube or flask.
whitw to whitish gray
Bacillus subtilis E. coli Pseudomons fluorenscens streptococcus faecilis most bacteria can grow on nutrient agar
Probably yes, E. coli grows much faster than most microbes
Bacillus
For colony appearance u want to describe what it looks like on agar not under a microscope. In terms of color it depends on what type of growth medium u are using but if it's just plain nutrient agar: Pigmentation: Colorless Opacity: Translucent Form: Circular Margin: entire Surface: Smooth Elevation: Raised.
E. coli is actually green on tryptic soy agar. The agar itself is a white, amber color.
* Colonies are circular, 2-3 nm in diameter with a smooth shiny surface when grown on nutrient agar,milk agar or blood agar for 24 h at 37 degrees * Colonies are often pigmented,though a few strains are unpigmented Got the answers from here http://www.geocities.com/SouthBeach/Port/3008/staphyl.html
Sheep blood agar inhibits gram negative bacteria. E. coli is gram negative.
The intended use of Nutrient Agar with MUG is for detecting an enumerating E. Coli in water. This lab wasn't looking specifically and only for e.coli. It was looking for other types of coliforms as well. this is my best guess
P. morabilis is characterized by its swarming ability. On a nutritive media plate the organism will swarm the entire plate due to its hyper mobility from its peritrichious flagella. Colonies can generally be seen to be opaque with irregular borders and have a smooth texture.
Escherichia coli and many others
It's not easy to differentiate the pigment of p aeruginosa on pseudomonas agar P from the pigment of E. coli on MacConkey agar without referring to the colors, but it can be done based on the structure of the p aeruginosa.