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1. chromophore is an isolated covalently bonded group which characteristically absorbs in the visible range of electromagnetic radiation. But fluorophore is any group capable of absorbing energy and emitting radiation with a longer wavelength.

2. Chromophores may have a overlapping ground singlet and excited singlet state but a fluorophore must have non overlapping S0 and S1 orbitals.

3. Chromophores can't emit energy but fluorophores can.

4. Chromophores are of 2 main types, extended π bond system and metal complex chromophore. Fluorophores are of 2 types, extrinsic and intrinsic.

5. Chromophores can't be quenched but fluorophores can.

6. Chromophores' absorption is not temperature dependent but fluorophores' fluorescing capacity is temperature dependent.

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Q: What is the difference between chromophore and fluorophore?
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