0
Acetone is used for decolouring method, washing away the iodine-crystal violet complex formed in gram negative bacteria. so that the gram negative bacteria can be then stained with safranin or fuchsin.
Wiki User
∙ 13y ago
Wiki User
∙ 12y agoThe alcohol is a decolorizing agent, and rids slide of the crystal violet if it's not retained by the cells. It must be applied sparingly and quickly removed since it can completely remove all of the crystal violet (or whichever primary stain you are using)
Wiki User
∙ 11y agoAcetone alcohol is the decolorizer. It removes the primary stain (crystal violet) from the cell wall of Gram negative cells, allowing them to take up the secondary stain (safranin), resulting in different colored cells based on their cell wall composition
Wiki User
∙ 9y agoThe acetone is used as the decoloring part of gram staining. Either the acetone will wash away the previous step of iodine and crystal violet or it will not wash off the crystals.
Wiki User
∙ 10y agoAcetone is added to remove the violet stain. Then the last stain is added. If the bacteria is a Gram+, the acetone can not remove the violet.
Wiki User
∙ 10y agowhat is the function of ethyl alcohol
Wiki User
∙ 11y agoto fix the bacteria
Add your answer:
Earn +20 pts
What are the reagents and steps min a Gram stain?
The steps in Gram staining are:1. crystal violet added to the smear2. iodine, the mordant (this fixes the violet)3. a decolorizer made of acetone and alcohol4. safranin, the counterstainIf the cell is Gram +, the decolorizer can not remove the violet. If it is Gram -, the decolorizer can remove the violet and the cell can be then colored with the dye, safranin.Bacteria are grouped in 4 groups by Gram stain:Gram-positive, the cell wall retains crystal Violet.Gram-negative, the cell wall does not retain crystal Violet.Graham not reactive, no staining whatsoever.Graham variable, uneven staining.
What bacterial staining technique is it when a basic dye is utilized to stain bacterial cells?
A basic dye used in gram staining is crystal violet.
Are there endospores in pseudomonas aeruginosa gram staining?
yes
What are four possible results of a gram stain?
The four possible results after Gram staining are Gram positive, Gram negative, Gram variable and Gram in-determinant.
Why gram staining is classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Function of iodine in staining?
iodine act as a mordant..on the gram positive bacteria which got really thick and abundence of peptidoglycan layer, the crystal violet will fix to the peptidoglycan layer..meanwhile in gram negative bacteria which is lack of peptidoglycan layer, the alcohol or acetone will wash it away
Is gram staining related to flagella?
No. It is a staining on the cell itself.
Who developed Gram staining?
Gram staining was devised by Hans Christian Gram of Denmark in the 1800s. (1853-1938)
What are the reagents and steps min a Gram stain?
The steps in Gram staining are:1. crystal violet added to the smear2. iodine, the mordant (this fixes the violet)3. a decolorizer made of acetone and alcohol4. safranin, the counterstainIf the cell is Gram +, the decolorizer can not remove the violet. If it is Gram -, the decolorizer can remove the violet and the cell can be then colored with the dye, safranin.Bacteria are grouped in 4 groups by Gram stain:Gram-positive, the cell wall retains crystal Violet.Gram-negative, the cell wall does not retain crystal Violet.Graham not reactive, no staining whatsoever.Graham variable, uneven staining.
Why gram staining classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Why is Gram staining classified as differential staining?
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Why is gram staining important?
It tells the microbiologist/clinician facts about the cell wall construction of the bacterium. This then indicates which antibiotics to prescribe if you are trying to get rid of a bacterial infection.
Why is safrinin used in gram staining?
as a couterstain
What is staining for candida albicans?
gram positive
What are the colony characteristics and Gram staining of bacillus circulans?
Gram Positive Rods
What function of lugol's iodine in gram staining?
fixing the stain so that the first dye which is the crystal violet will not be washed away during rinse process.
Is gram staining special staining?
yes it is a special staining like other types....its importance is that one can identify the type of bacteria........
