0
What is the restriction site of the restriction enzyme TaqI?
Anovia
TaqI's restriction site is:
TCGA
AGCT
Wiki User
∙ 13y ago
Add your answer:
Earn +20 pts
What is opened by a restriction enzyme?
A highly specific 'active site'.
What is the restriction site of the restriction enzyme Hae III?
The restriction site of Hae III is GGCC. It cuts between the G and the C. This produces blunt ends.
How restriction enzyms are use in DNA recommination research?
they used to cut the DNA at the specific site. For example: BamHI is a restriction enzyme that cuts between the given recognition site:
What is the restriction site to restriction enzyme Hae III?
Hae III cuts at the site GGCC. It creates blunt ends - meaning a clean cut. This is found between the G and C.
Can Restriction Enzymes be used on all DNA?
Restriction enzymes cut DNA at sites called restriction sites on the DNA. These restriction sites are specific sequences of 6 - 8 nucleotide bases. Restriction enzymes can be used on all types of DNA. If the DNA is cut by a certain restriction enzyme, then we know that the DNA contained the restriction site. This sort of an experiment is called restriction site analysis
What is HinF1 site in DNA sample?
restriction enzyme site
What is opened by a restriction enzyme?
A highly specific 'active site'.
What is the restriction site of the restriction enzyme Hae III?
The restriction site of Hae III is GGCC. It cuts between the G and the C. This produces blunt ends.
What is the term that describes the sequence of DNA that a restriction enzyme finds and cuts?
Template Sequence
What is the best explanation for why a restriction enzyme does not cut the DNA of the cell that produces it?
The cell's DNA does not contain the restriction site.
What is a DNA restriction site?
The restriction site is a sequence of DNA that is recognized by an endonuclease, or a protein that cuts DNA, as a site at which the DNA is to be cut. This cutting happens when restriction enzyme cleaves nucleotides by hydrolyzing the phosphodiester bond between them.
An enzyme that cuts double-stranded DNA at specific nucleotide sequences?
Such an enzyme is called a restriction endonuclease
Why restriction enzyme cannot cut its own DNA?
Restriction enzymes are produced by bacteria to help destroy foreign, invading DNA, such as the DNA of bacteriophage (a virus that infects bacterial cells). Every restriction enzyme comes with a methylase enzyme, or more specifically, a DNA methyltransferase. The methylase enzyme methylates (adds a methyl group) to the restriction endonuclease site on the cell's own DNA, which protects the sites from the restriction enzyme so that it does not degrade its own DNA.
How restriction enzyms are use in DNA recommination research?
they used to cut the DNA at the specific site. For example: BamHI is a restriction enzyme that cuts between the given recognition site:
What is the restriction site to restriction enzyme Hae III?
Hae III cuts at the site GGCC. It creates blunt ends - meaning a clean cut. This is found between the G and C.
What determines how DNA will be cut by a restriction enzyme?
Restriction enzymes cut DNA at specific sites called restriction sites. These restriction sites are typically 6 - 8 nucleotides in length and have a defined set of nucleotide bases. For example, the restriction enzyme Eco R1 cuts at the site: AGGTTC. Therefore, if the target DNA contains the above sequence, Eco R1 is able to cut it within the restriction site. Hence, by looking into the target site and which restriction enzymes are being used, on can make an accurate estimate of where the target DNA will be cut
Can Restriction Enzymes be used on all DNA?
Restriction enzymes cut DNA at sites called restriction sites on the DNA. These restriction sites are specific sequences of 6 - 8 nucleotide bases. Restriction enzymes can be used on all types of DNA. If the DNA is cut by a certain restriction enzyme, then we know that the DNA contained the restriction site. This sort of an experiment is called restriction site analysis
