Uñderstand by example: Bacteria that have pSC 101 plasmid, this plasmid have antibiotic resistant gene for tetracycline.when gene of interest in attached to plasmid to produce clone to get many genes of interest, it is placed in a medium contaning tetracycline for culturing bacteria(bactria made colonies which would separate out and remain safe because of they have resistance against tetracycline while other phothogen donot häve)
no
Usually recombinant DNA is packaged in a plasmid that contains a marker gene. This marker can be an antibiotic resistance gene (NPTII for Kanamycin) or a gene that enables the plant to synthesise an amino acid. For antibiotic resistance the cells are grown on a medium that contains the antibiotic. The ones that grow have the marker gene. Sometimes the cells are transformed with a mixture of plasmids, some with the target gene and some without. The LAC-operon is used to select the cells that have the gene inserted. The gene-insertion inactivates the LAC-Z gene. Cells grown on X-gal plates will be blue, unless there's a transgene present. So white colonies have the transgene.
If antibiotic resistance is added to the gene being cloned, antibiotics can be used to isolate the transformed bacteria (ones with the gene being cloned) by killing off all non-transformed bacteria, that don't have the antibiotic resistance. There is a chance that the non-transformed bacteria can mutate to develop antibiotic resistance.
genetic marker
The plasmid that contains foreign DNA is engineered to also carry an antibiotic resistance gene. This antibiotic resistance gene codes for a protein that is able to inactivate an antibiotic thus keeping the cell alive. In the absence of the antibiotic resistance gene, the cells would not survive when exposed to an antibiotic. After transfection (the process of inserting the plasmid carrying the foreign gene into cells), the cells are gown in media containing an antibiotic. Cells that contain the plasmid (and therefore contain the antibiotic resistance gene) are able to survive in this medium. Cells that do not contain the plasmid (and therefore lack the antibiotic resistance gene) do not survive in this medium. The process described above is called selection
gene of interest 2.molecular scissor 3.molecular carrier or vector 4.expression system .These are the requirments for recombinant DNA technology.
Genetic Marker
false
false
Plasmid
The prophage takes an antibiotic resistance gene with it and is packaged with the newly synthesized viral DNA.
The role of cloning host in RDT is to serve as the host cell under culture, in which the designed gene is subjected to produce recombinant protein.