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Walther Flemming made use of aniline dyes to find a structure in the cell nucleus which strongly absorbed basophilic aniline dyes, which he named chromatin (later called chromosomes). Basophilic is a technical term used by histologists. It describes the microscopic appearance of cells and tissues, as seen down the microscope, after a histological section has been stained with a basic dye. The most common such dye is haematoxylin.
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What is the secondary stain in gram stain procedure?
It is crystal violet & stains all cells purple.
What advantages of negative stain?
Since heat fixation is not required and cells are not subjected to the distorting effects of chemicals and heat, their natural size and shape can be seen. Also, it makes it possible to view hard to stain bacteria such as spirilli.
Why is decolorization necessary when performing a gram stain?
During the procedures of a gram stain, decolorization is necessary to remove any stain or color from the gram negative cells. When a dye is used to stain gram positive cells, both gram positive and gram negative cells retain color. Mordant is used to bind the original stain to gram positive cells so when decolorizer is used they retain color. After the mordant has been used a decolorizer is used to wash away colo in gram negative cells. Counterstains are used to stain gram negative cells to better visualize contrasting cells. An example of a decolorizer that works well is ethanol.
Why does the counterstain not change the color of all of the cells is it because the primary stain repels or masks the secondary stain?
In a gram stain the primary stain is crystal violet. Iodine then sets that dye into the gram positive cells while alcohol washes out the crystal violet from the gram negative cells. Then safranin, which is the counterstain in a gram stain, is used to dye the rest of the bacteria. This is the example I can give you of why a counterstain does not change the look in all the cells. Though safranin stains all the cells, the gram positive cells that were dyed purple from crystal violet don't look pink - only the gram negative do.
Is water a stain remover?
Yes, but not for all stains.
What can you gram stain living cells and look at the living cells under the microscope?
yes, but only with certain stains because some stains are toxic to live organisms.
What is the purpose of staining an unknown organism?
By doing differential stains on an unknown organism, you can learn more about that organism. One of the most helpful stains would be the Gram stain. The gram stain will differentiate from Gram positive and Gram negative cells, narrowing your bacteria down a lot. Other stains include: Acid-Fast stain, Capsule stain, Endospore stain, and PHB stain.
What is the secondary stain in gram stain procedure?
It is crystal violet & stains all cells purple.
What advantages of negative stain?
Since heat fixation is not required and cells are not subjected to the distorting effects of chemicals and heat, their natural size and shape can be seen. Also, it makes it possible to view hard to stain bacteria such as spirilli.
Why is decolorization necessary when performing a gram stain?
During the procedures of a gram stain, decolorization is necessary to remove any stain or color from the gram negative cells. When a dye is used to stain gram positive cells, both gram positive and gram negative cells retain color. Mordant is used to bind the original stain to gram positive cells so when decolorizer is used they retain color. After the mordant has been used a decolorizer is used to wash away colo in gram negative cells. Counterstains are used to stain gram negative cells to better visualize contrasting cells. An example of a decolorizer that works well is ethanol.
What is advantage of using stain?
Using stains on slides help show some details that may be unclear otherwise, especially in cells.
Why does mustard stain?
Mustard stains because it is a dye stain ,which makes it difficult to get out.
When is Negative stain used?
it is used in diagnostic microscopy (to visualize the edges of protein complexes, macromolecules and cells in suspension).
Why do you use iodine to stain a cell?
Iodine stains starch contained in cells. Iodine is also used to distinguish between Gram negative and Gram positive bacteria. The Gram stain contains iodine.
What structure does aceto-orcein stain in plant cells or animal cells?
Methylene blue stains both the nucleus and the cytoplasm of eukaryotic cells. The nucleus typically stains a deeper color. Its purpose is to make cells show up against their background. Methylene blue is also used to stain DNA or RNA after electrophoresis. It can be purchased at most aquarium shops for under 10 dollars. In some cases food dye might be suitable.
Why does the counterstain not change the color of all of the cells is it because the primary stain repels or masks the secondary stain?
In a gram stain the primary stain is crystal violet. Iodine then sets that dye into the gram positive cells while alcohol washes out the crystal violet from the gram negative cells. Then safranin, which is the counterstain in a gram stain, is used to dye the rest of the bacteria. This is the example I can give you of why a counterstain does not change the look in all the cells. Though safranin stains all the cells, the gram positive cells that were dyed purple from crystal violet don't look pink - only the gram negative do.
What is supra vital stains?
methylene blue and crystal violet, supravital stains are essential to identify Heinz bodies in G6PD deficiency patients. There are more but I do know another is brilliant cresyl. Supravital stains stain living cells. It also stains many other types of inclusions along with identifying Hemoglobin H, which is found in alpha thalassemia.
