Why are bananas used in DNA extraction?
Maybe because bananas are annual kind of plant, which means they can survive in all year round in both rainy season and summer season and it's easy to produce unlike to the other plant to our surroundings. On the other hand, bananas have a juicy stem or body, it has a complete structures of genetic information and besides banana can reproduce itself, without mating from the other plant nor from the other species of banana.(asexual reproduction).
4 people found this useful
liquid nitrogen (-170 C) which it freeze the tissue to become fragile to be a fine powder which increase the surface area of extraction, and the very low temperature prevent D…Nase activation * the primary function of this step is to lyse the cells
MgCl2 is used to preserve the integrity of membrane system by counteracting the fixed negative charges of membrane phospholipid. Depending on what you want to extract, it trie…s to protect the component you are interested in (DNA/RNA/red blood cells, etc) from being lyzed by broken-open lysosome for instance.
Because it is less dense than the alcohol and make it float
No-DNA exists in a microscopic level, you can't obtain it using a blender.
Salt helps in breaking the protein chains around the nucleic acids
It helps break the nuclear membrane of the cell. Detergent containing the compound SDS ( sodiumdodecyl sulfate) is used to break down and emulsify the fat and proteins that… make up a cell membrane.
Although it it a bad idea to use something as unregulated as dish washing liquid for DNA isolation, the scientific principle is that proteins denature (or break up) in the pre…sence of a detergent. Denatured proteins can then be separated from the remainder of the cellular contents by centrifugation. This leaves a supernatant containing mainly nucleic acids (DNA and RNA) Dish washing liquid is unregulated because we do not know exactly what is in there since it is a commercially available product. There could be other ingredients (like fragrances and other appearance enhancers) that might interfere with DNA isolation. Laboratory grade detergents like SDS and CTAB are used for DNA isolation
Although DNA can be isolated from a variety of sources, there are some general considerations for choosing a cell. They are: . The cell should have a nucleus containing DNA .… The cell should be viable (alive). Although DNA can be isolated form dead cells as well, they are sometimes fragmented in dead cells therefore a viable cell is preferable for good quality DNA . The cell should not be infected with a virus. If an infected cell is used, the viral genetic material will conaminate the source DNA
Detergent is a dehydrating agent that allows the DNA to precipitate. Precipitated DNA can then be separated from the other components through a process called centrifugation.
It breaks down the Cell Membrane, making it easier for the DNA to come through. Good Luck :)
In Botany or Plant Biology
When extracting DNA from a banana, the DNA must release from the cell by breaking apart or lysing the cellular and nuclear membranes. Lysing in this case is the act of breakin…g open the cell membranes to expose the contents. This is performed by mashing the banana and adding a detergent/salt solution.
According to me, we use alcohol because DNA is insoluble inalcohol, it aggregates together, giving a pellet in centrifugal andwe can see a precipitated DNA with naked eyes (th…at we suppose tosee in experiment i.e DNA extraction)....
In Bananas and Plantains
The reason why isoproponal is used in DNA extraction is because it is stronger at precipitating the DNA. DNA is also insoluble in isoproponal and is cold to help the DNA retai…n its shape.
the benefit to extract DNA from banana IS TO PRODUCE NEW AND MORERAPID POPULATION OF FRUITS