Because the peptone iron agar is used to detect ANAEROBIC bacteria. If you stab it deep into the agar you allow the bacteria to grow in the absence of oxygen. If you only inoculated the surface the bacteria wouldn't grow.
peptone agar is used to show the production of hydrogen sulfide
peptone, yeast extract,meat extract..
culture is stabbed deeply into the agar using an inoculating needle....
inoculation needle
Peptone and beef extract
A inoculating loop is used for transfers from culture plates to culture tubes instead of the inoculating needle because the needle could puncture the agar in tube. The loop is much easier as well to get liquid amount into the tube.
Peptone is the simplified source of protein and can be readily uptake by the microorganism. In nurtient agar it is the basic component/nutrient after which beef or yeast extract is used which are supplementary nutrients.
Peptone glucose agar (PGA) medium
This is important to prevent the inoculating needle from becoming stuck in the agar, taking out pieces of agar while trying to remove the instrument. This agar will get into the inoculum when sterilizing the needle on the flame, causing contamination to your sample.
thats a good question but unforunately i dont now the answer
Beef extract for carbohydrates Peptone for protein and NaCl as salt
It is called a streak plate, inoculating the agar plate with a zig-zag motion.