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Why is acid alcohol rather than ethyl alcohol used as a decolorizing agent in the acid fast staining experiment?
Wiki User
∙ 9y ago
Alcohol is a term used for any O-H group that is attached to a carbon.
Perhapes the alcohol is found in a Safranin stain. I hope I have this right but if alcohol was used as the decolorizing agent, it may wash out too much stain to get a good view in an oil emersion microscope.
Wiki User
∙ 13y ago
Wiki User
∙ 9y agoAcid alcohol is used as a decolorizing agent in the acid fast staining procedure because acid binds to it and the stain sticks but it will decolorize non acid fast organisms. Ethyl alcohol will decolorize everything acid fast and non acid fast.
Wiki User
∙ 13y agoThe mycolic acids in the cell walls of acid-fast bacteria make them resistant to acid-alcohol.
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If you conduct an experiment and draw conclusions that are based on your beliefs rather than facts you are using?
Objective reasoning
What pieces of information gathered through and experiment called?
Depending on the experiment, scientists collect an array of information. The information, also known as data, can be put into two categories...Qualitative: descriptions rather than measurements.OrQuantitative: recorded measurements.
Why is it important than an experiment include a control group?
Depending on the experiment, expectation can be very powerful. In a medical experiment, a control group can help determine whether the drug being tested is responsible for the result rather than expectation or something else. For a study of a rare occurrence, a control group may help an experimenter determine whether the outcome is due to chance.
Does isobutyl alcohol dissolve sugar?
Yes, sugar is soluable in alcohol. I am assuming you mean drinking alcohol although it is soluable in all organic alcohols. The hyrdoxy (-OH) groups on both the sugar and the alcohol allow for hydrogen bonding, making sugar very soluable in alcohol. Hydrogen bonds are also the reason sugar is so soluable in water.
What is the control in a expirment?
The control in an experiment is what you are changing, rather like the independent variables in a function in math. You may be doing an experiment regarding, say, the amount of light reflected off a surface. The control here is the material you are using to reflect the light. You are CONTROLLING it. The luminosity of the reflection is the reading you get, so it's your result. Just remember the control is what you are able to change to affect the outcome.
What would be the result of the gram stain if acid alcohol is used as a decolorizer rather than the gram decolorizer?
alcohol will remove the lipid layer of the G- cells and the outer membrane. Edit: Ethyl alcohol is used as a decolorization step. Without using ethyl alcohol the gram-negative cells will still remain colored. H2O may dilute the cells or wash them off the slide.
How do i find the sum of outcomes in probability?
Select an experiment that has a random result rather than one that is deterministic. The result of the experiment is the outcome of the probabilistic experiment.
Would you rather use alcohol or Mercury in a thermometer?
Alcohol. Mercury is very toxic.
How does alcohol affect the cerebrum?
Alcohol does not affect the cerebrum...rather it affects the cerebellum of the brain.
Does alcohol cause the bladder to become enlarged?
no,rather it neutralises it.
Why does drinking through a straw get you more drunk?
Because you avoid temporary and permanent teeth staining, eliminate wine-staches, and any lipstick and lipgloss stays on your mouth (not on the wine glass). And make sure if you use a straw for drinking wine, its a WineStraw!
What is rationale and methodology?
Methodology is a description of what was done in the experiment - whatever the experiment was. The rationale was the reason for doing it in the way that you did, rather than any other way.
Does aftershave have a use by date?
depends on brand, but if it contains alcohol it would loose potency rather quickly due to the evaporation of the alcohol
Why would you use alcohol thermometer rather then a mercury thermometer in the north pole?
because it would turn to ice and alcohol wouldn't
What is the solute in 60.0 mL of ethyl alcohol and 30.0 mL of methyl alcohol?
In this case the solute is methyl alcohol and solvent is ethyl alcohol. When two liquids mix, they are not usually referred to as "soluble", but rather are referred to as "miscible".
What is a person called whose knowledge is obtained from trials observation and experiment rather than theory?
Empiric
What is the benefit of conducting an experiment rather than using a different type of study?
accutate and realistic results
