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Would you prepare crystal violet

Updated: 8/21/2019
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What color would the bacterial cell have when viewed microscopically after the treatment with ammonium oxalate crystal violet?

Violet


What is the secondary stain in gram stain procedure?

It is crystal violet & stains all cells purple.


What happens if grams iodine is not applied after staining with crystal violet?

If the iodine in the Gram's stain was omitted, the crystal violet which enters the cell will not be able to form the Crystal Violet Iodine (CVI) complex. The CVI complex molecule has a larger size than that of Crystal violet molecule and hence Gram +ve bacteria are able to retain the stain. If iodine were omitted, even Gram+ve cells would get decolorised by Alcohol treatment and would be seen as Gram-ve cells.


What is gentian violet?

Traditional antifungal drugs such as.gentian violet (also known as crystal violet)


What is crystal violet iodine complex?

In Gram staining procedure during bacterial staining , iodine forms a complex with crystal violet stain which stains Gram positive bacteria blue to violet .


What is the extinction coefficient of crystal violet?

balls


Why is Iodine used in Gram staining?

Iodine is added as a mordant to enhance crystal violet staining by forming a crystal violet-iodine complex.


If iodine step was amitted how would gram negative cells appear?

Iodine work as modrant to crystal violet if the iodine step will be emitted then the crystal violet will not be able to show its effect and all cells will appear pink when counter stain with saffranin


Crystal violet acid-base indicator?

Yes it is.


Function of crystal violet?

Crystal violet is a hexamethyl also known as methyl violet 10B This is much darker than 2B, and often darker than 6B. It is used in biological stainang particularly gram staining together with safranin and iodine. Crystal violet was also used to treat strept throat. Doctors used to swab it on the back of your throat.


How would removing the crystal violet from MacConkey agar alter the sensitivity and specificity of the medium?

It would allow growth of Gram-positive cocci.


Why do you add crystal violet stain in simple staining method?

Because violet dye is the least expensive.