3T3 cells come from a cell line established in 1962 by two scientists then at the Department of Pathology in the New York University School of Medicine, George Todaro and Howard Green. The 3T3 cell line has become the standard fibroblast cell line. Todaro and Green originally obtained their 3T3 cells from Swiss mouse embryo tissue.
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Nomenclature
The '3T3' designation refers to the abbreviation of "3-day transfer, inoculum 3 x 105 cells." This cell line was originally established from the primary mouse embryonic fibroblast cells that were cultured by the designated protocol, so-called '3T3 protocol'. The primary mouse embryonic fibroblast cells were transferred (the "T") every 3 days (the first "3"), and inoculated at the rigid density of 3 x 105 cells per 20-cm² dish (the second "3") continuously. The spontaneously immortalized cells with stable growth rate were established after 20-30 generations in culture, and then named '3T3' cells.
Uses
3T3 cells are often used in the cultivation of keratinocytes, with the 3T3 cells secreting growth factors favourable to these kinds of cells.
Drugs affecting 3T3 cells
Benzodiazepines such as temazepam have been found to inhibit Swiss 3T3 cells.[1]
References
- Todaro GJ , Green H . Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J. Cell Biol. 17: 299-313, 1963. Fulltext.
- Nikon MicroscopyU Digital Video Gallery: 3T3 Cell Motility - a set of films of 3T3 cells in culture
Notes
- ^ Wang JK, Morgan JI, Spector S (February 1984). "Benzodiazepines that bind at peripheral sites inhibit cell proliferation" (PDF). Proc Natl Acad Sci USA 81 (3): 753–6. doi:. PMID 6322168. http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=344914&blobtype=pdf.
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