1 Avoid contamination so be very careful that you avoid spillover from adjacent wells. 2 Don't allow the plate to dry out. 3 Make sure that all incubations are carried out in 100% humid conditions. (wrapping the covered plate in a wet paper towel and then placing in a sealed bag works) 3 Wash the plate well and carefully with PBS-tween between steps at least 3 times. 4 Use fresh buffers all at the correct pH. 5 Don't use any azide in your buffers if you use peroxidase conjugates. There are more but it takes experience to do an ELISA test well.

You can spell the phrase "Lunch in TARDIS: booboo!" and several thousand less-apt phrases.

The longest single shorter words are consolidations, chlorinations, discoloration, and subordination.