A. spinosa may refer to:
- Absidia spinosa, a fungus
- Acanthemblemaria spinosa, a blenny
- Acanthus spinosa, a flowering plant
- Acanthopleura spinosa, a species of chiton, a primitive marine mollusk
- Adejeania spinosa, a tachinid fly
- Adenia spinosa, a passionflower
- Agama spinosa, a small, long-tailed, insect-eating lizard
- Agathosma spinosa, a small shrub
- Amorphoscelis spinosa, a praying mantis
- Almenia spinosa, an arthropod
- Ancylotrypa spinosa, a wafer trapdoor spider
- Anotheca spinosa, or Rana De Corona, a frog
- Aphaenogaster spinosa, a myrmicine ant
- Arachnura spinosa, an orb-weaving spider
- Aralia spinosa, a woody plant
- Argania spinosa, or Argan, a tree
- Atelidea spinosa, a long jawed spider
- Auximella spinosa, a tangled nest spider
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