AEIOU can refer to:
- A.E.I.O.U., a device used by the Habsburgs
- aeiou Encyclopedia
- Aeiou may also refer to a cartoon character featured in Muse magazine.
- "a, e, i, o, u" is a traditional list of vowel letters in the Roman alphabet.
- A.E.I.O.U. - an 2005 Album by Polish Hip-Hop group Sistars
- AEIOU is a graphic novel by cartoonist Jeffrey Brown.
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