Affinity label
Oxford Dictionary of Biochemistry:
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affinity label
an active-site-directed irreversible inhibitor of an enzyme, antibody, or other protein. It is a chemically reactive compound that resembles a substrate or other specific ligand and bonds covalently to the active site or specific site on the protein. The affinity labelled groups can then be identified by fingerprinting and thus reveal the composition at the active site. It is sometimes termed Trojan horse inhibitor.
| affinity gel, affinity electrophoresis, affinity electrode | |
| affinity matrix, affinity precipitation, affinity support |
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Affinity label
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Affinity label is a molecule that is similar in structure to a particular substrate for a specific enzyme. It is considered to be a class of enzyme inactivators. These molecules covalently modify active site residues in order to elucidate the structure of the active site.
These types of compounds are highly reactive and usually alkylate nucleophilic amino acids present in the enzyme. They are often indiscriminant in their action, therefore their usefulness is limited as they are non-selective and therefore inherently toxic.
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Oxford Dictionary of Biochemistry
Oxford University Press. Oxford Dictionary of Biochemistry and Molecular Biology © 1997, 2000, 2006 All rights reserved.
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Mentioned in
Slim & Slam [Affinity]
(1938 Album by Slim & Slam)
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(1951 Album by Jess Stacy/Ralph Sutton)
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(1956 Album by Betty Roché)
Swinging Tenors
(1944 Album by Bud Freeman)
Out of the Shadow
(1982 Album by Herbie Nichols)
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