AUI may stand for:
- Ethernet's Attachment Unit Interface, a 15-pin D-connector
- The conlang aUI (language)
- The National Rail code for Ardlui railway station, United Kingdom
- Associated Universities, Inc., the corporation that operates the National Radio Astronomy Observatory (NRAO)
- Amiga User International, a monthly magazine dedicated to the Amiga computer
- AUI (language)
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