Of or relating to a nucleotide sequence that is complementary to a sequence of messenger RNA. When antisense DNA or RNA is added to a cell, it binds to a specific messenger RNA molecule and inactivates it.
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Of or relating to a nucleotide sequence that is complementary to a sequence of messenger RNA. When antisense DNA or RNA is added to a cell, it binds to a specific messenger RNA molecule and inactivates it.
| It has been suggested that this article or section be merged into Sense (molecular biology). (Discuss) |
Antisense molecules interact with complementary strands of nucleic acids, modifying expression of genes.
Some regions within a double strand of DNA code for genes, which are usually instructions specifying the order of amino acids in a protein along with regulatory sequences, splicing sites, noncoding introns and other complicating details. For a cell to use this information, one strand of the DNA serves as a template for the synthesis of a complementary strand of RNA. The template DNA strand is called the transcribed strand with antisense sequence and the mRNA transcript is said to be sense sequence (the complement of antisense). Because the DNA is double-stranded, the strand complementary to the antisense sequence is called non-transcribed strand and has the same sense sequence as the mRNA transcript (though T bases in DNA are substituted with U bases in RNA).
DNA strand 1: sense strand
DNA strand 2: antisense strand (copied to)→ RNA strand (sense)
Many forms of antisense have been developed and can be broadly categorized into enzyme-dependent antisense or steric blocking antisense.
Enzyme-dependent antisense includes forms dependent on RNase H activity to degrade target mRNA, including single-stranded DNA, RNA, and phosphorothioate antisense. The R1 plasmid hok/sok system is an example of mRNA antisense regulation process, through enzymatic degradation of the resulting RNA duplex. Double stranded RNA acts as enzyme-dependent antisense through the RNAi/siRNA pathway, involving target mRNA recognition through sense-antisense strand pairing followed by target mRNA degradation by the RNA-induced silencing complex (RISC).
Steric blocking antisense (RNase-H independent antisense)[1] interferes with gene expression or other mRNA-dependent cellular processes by binding to a target sequence of mRNA and getting in the way of other processes. Steric blocking antisense includes 2'-O alkyl (usually in chimeras with RNase-H dependent antisense), peptide nucleic acid (PNA), locked nucleic acid (LNA) and Morpholino antisense.
Antisense nucleic acid molecules have been used experimentally to bind to mRNA and prevent expression of specific genes. Antisense therapies are also in development; in the USA, the Food and Drug Administration (FDA) has approved a phosphorothioate antisense oligo, fomivirsen (Vitravene), for human therapeutic use.
Cells can produce antisense RNA molecules naturally, which interact with complementary mRNA molecules and inhibit their expression.
Weiss, B. (ed.): Antisense Oligodeoxynucleotides and Antisense RNA : Novel Pharmacological and Therapeutic Agents, CRC Press, Boca Raton, FL, 1997.
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Dansk (Danish)
adj. - anti-sense
Nederlands (Dutch)
het functioneren van genen tegenhoudend (biologie)
Français (French)
adj. - contre-sens
Deutsch (German)
adj. - in Komplementärsequenz mit Genstück, die Genfunktion hemmend
Ελληνική (Greek)
adj. - (βιολ.) (για γονίδιο) ανάστροφος
Italiano (Italian)
controsenso
Português (Portuguese)
adj. - sem sentido
Русский (Russian)
(о цепи фНК) комплементарная, не несущая информацию о структуре
Español (Spanish)
adj. - antígeno
Svenska (Swedish)
adj. - som hämmar genfunktionen
中文(简体) (Chinese (Simplified))
反意识的
中文(繁體) (Chinese (Traditional))
adj. - 反意識的
العربيه (Arabic)
(صفه) غير منطقي, رفض المنطق
עברית (Hebrew)
adj. - של גן המוחדר לAND ומשמש בהנדסה גנטית לוויסות ביטוי גנטי לתכונה
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![]() | Dictionary. The American Heritage® Dictionary of the English Language, Fourth Edition Copyright © 2007, 2000 by Houghton Mifflin Company. Updated in 2007. Published by Houghton Mifflin Company. All rights reserved. Read more | |
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