Bacterial display

Bacterial display (or bacteria display or bacterial surface display) is a protein engineering technique used for in vitro protein evolution. Libraries of polypeptides displayed on the surface of bacteria can be screened using flow cytometry or iterative selection procedures (biopanning).

Competing methods for protein evolution in vitro are phage display, ribosome display, yeast display, and mRNA display.

References

• Francisco, J. A.; Campbell, R.; Iverson, B. L.; Georgiou, G. (1993). "Production and Fluorescence-Activated Cell Sorting of Escherichia coli Expressing a Functional Antibody Fragment on the External Surface". Proc. Nat. Acad. Sci. U.S.A. 90: 10444–48. doi:10.1073/pnas.90.22.10444. 
• Georgiou, G.; Stathopoulos, C; Daugherty, PS; Nayak, AR; Iverson, BL; Curtiss R, 3rd (1997). "Display of heterologous proteins on the surface of microorganisms: From the screening of combinatorial libraries to live recombinant vaccines". Nat. Biotech. 15 (1): 29–34. doi:10.1038/nbt0197-29. PMID 9035102. 

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